Immunolocalization of exoglycoproteins (ependymins) in the goldfish brain

Exoglycoproteins (X-GPs) are a group of very abundant soluble glycoproteins in the goldfish brain. Immunostaining with polyclonal antisera to X-GPs revealed consistent perinuclear staining in the cells of the inner and intermediate layers of the leptomeninx, which is homologous to the pia-arachnoid....

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Bibliographic Details
Published in:Neurochemical research 1994-11, Vol.19 (11), p.1401-1412
Main Authors: LAKOS, S. F, THORMODSSON, F. R, GRAFSTEIN, B
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Western
Brain - cytology
Brain Chemistry
Capillaries - cytology
Carassius auratus
Cerebral circulation. Blood-brain barrier. Choroid plexus. Cerebrospinal fluid. Circumventricular organ. Meninges
Cerebral Ventricles - cytology
Fundamental and applied biological sciences. Psychology
Glycoproteins - analysis
Goldfish
Immune Sera
Immunohistochemistry
Lymphocytes - cytology
Nerve Tissue Proteins - analysis
Neurons - cytology
Superior Colliculi - blood supply
Superior Colliculi - cytology
Vertebrates: nervous system and sense organs
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Summary:Exoglycoproteins (X-GPs) are a group of very abundant soluble glycoproteins in the goldfish brain. Immunostaining with polyclonal antisera to X-GPs revealed consistent perinuclear staining in the cells of the inner and intermediate layers of the leptomeninx, which is homologous to the pia-arachnoid. Immunolabelling was also prominent in the outer wall of capillaries, and in a variable population of 10-12 microns granular cells that appeared mainly near the ventricles and occasionally within the ventricles or under the meninges. In some cases, small and medium-sized lymphocytes were immunostained. Lymphocytes were sometimes associated with the granular cells, which may be hematogenous cells in transit toward the ventricles. The choroid plexus, saccus dorsalis, the roof of the third ventricle and Reissner's fiber showed strong immunostaining. The localization of the X-GPs suggests that they may contribute to maintenance of the blood-brain barrier or to regulation of immune function within the brain.
ISSN:0364-3190
1573-6903
DOI:10.1007/BF00972469