Use of nondenaturing silver-stained polyacrylamide gel analysis of polymerase chain reaction amplification products for the differential diagnosis of Leptospira interrogans infection

A 285-bp DNA fragment was amplified using the polymerase chain reaction from 38 Leptospira serovars of six different genomic species. The fragments amplified exhibited differential mobilities on nondenaturing polyacrylamide gels resulting from sequence-dependent conformational alterations. Leptospir...

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Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 1995-08, Vol.22 (4), p.343-348
Main Authors: Olivera, Marluce A.A., Caballero, Otávia L., Neto, Emmanuel Dias, Koury, Matilde Cota, Romanha, Alvaro J., Carvalho, Juçara, Hartskeerl, Rudy A., Simpson, Andrew J.G.
Format: Article
Language:English
Subjects:
Bacterial Typing Techniques
Bacteriological methods and techniques used in bacteriology
Bacteriology
Base Sequence
Biological and medical sciences
Diagnosis, Differential
DNA, Bacterial - analysis
Electrophoresis, Polyacrylamide Gel
Fundamental and applied biological sciences. Psychology
Humans
Leptospira interrogans - classification
Leptospira interrogans - genetics
Microbiology
Molecular Sequence Data
Polymerase Chain Reaction
Sensitivity and Specificity
Silver Staining
Weil Disease - diagnosis
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Summary:A 285-bp DNA fragment was amplified using the polymerase chain reaction from 38 Leptospira serovars of six different genomic species. The fragments amplified exhibited differential mobilities on nondenaturing polyacrylamide gels resulting from sequence-dependent conformational alterations. Leptospira interrogans serovars could be distinguished from those of other species on this basis.
ISSN:0732-8893
1879-0070
DOI:10.1016/0732-8893(95)00145-3