High-performance liquid chromatographic separation of fluorescent esters of hepoxilin enantiomers on a chiral stationary phase

Fluorescent anthryl (ADAM) derivatives of hepoxilins have been shown to possess good chromatographic properties affording good sensitivity for the high-performance liquid chromatographic analysis and detection of these compounds and related eicosanoids (12-hydroxyeicosatetraenoic acid) in biological...

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Bibliographic Details
Published in:Journal of chromatography. B, Biomedical applications Biomedical applications, 1995-10, Vol.672 (2), p.282-289
Main Authors: Demin, Peter, Reynaud, Denis, Pace-Asciak, Cecil R.
Format: Article
Language:English
Subjects:
8,11,14-Eicosatrienoic Acid - analogs & derivatives
8,11,14-Eicosatrienoic Acid - chemistry
8,11,14-Eicosatrienoic Acid - isolation & purification
Acetates
Anthracenes
Chromatography, High Pressure Liquid - methods
Chromatography, High Pressure Liquid - statistics & numerical data
Esters
Fluorescent Dyes
Stereoisomerism
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Summary:Fluorescent anthryl (ADAM) derivatives of hepoxilins have been shown to possess good chromatographic properties affording good sensitivity for the high-performance liquid chromatographic analysis and detection of these compounds and related eicosanoids (12-hydroxyeicosatetraenoic acid) in biological samples. We report herein the separation of all possible stereoisomers of hepoxilins A 3 and B 3 as their methyl esters as well as their ADAM ester and acetate derivatives on a cellulose trisdimethylphenylcarbamate chiral stationary phase (Chiracel OD) in the normal-phase mode. This methodology is important to address the mechanistic route of biosynthesis of these products.
ISSN:0378-4347
1572-6495
DOI:10.1016/0378-4347(95)00214-4