BCMAp: an integral membrane protein in the Golgi apparatus of human mature B lymphocytes

• Published in: International immunology 1995-07, Vol.7 (7), p.1093-1106
• Main Authors: Gras, Marie-Pierre, Laâbi, Yacine, Linares-Cruz, Gustavo, Blondel, Marie-Odile, Rigaut, Jean-Paul, Brouet, Jean-Claude, Leca, Gérald, Haguenauer-Tsapis, Rosine, Tsapis, Andréas
• Format: Article
• Language: English
• Subjects: B cell; B-Cell Maturation Antigen; B-Lymphocytes - chemistry; Cell Compartmentation; Fluorescent Antibody Technique; Golgi apparatus; Golgi Apparatus - chemistry; Golgi Apparatus - immunology; Humans; maturation; Membrane Glycoproteins - chemistry; Membrane Glycoproteins - genetics; Membrane Proteins - chemistry; Membrane Proteins - genetics; Microscopy, Confocal; Microsomes - chemistry; Protein Conformation; Receptors, Tumor Necrosis Factor; RNA, Messenger - chemistry; transmembrane protein; Tumor Cells, Cultured
• ISSN: 0953-8178; 1460-2377
• DOI: 10.1093/intimm/7.7.1093

BCMA is a human gene expressed preferentially in mature B lymphocytes as a 1.2 kb mRNA, which encodes a 184 amino acid peptide (BCMAp). The study of BCMA mRNA expression, using human malignant B cell lines characteristic of different stages of B lymphocyte differentiation, demonstrated that the BCMA mRNA is absent in the pro-B lymphocyte stage. It is expressed faintly at the pre-B cell stage and its expression increases with B lymphocyte maturation. Polyclonal antibodies were used to show, by cellular fractionation and immunoprecipitation, that BCMAp is a non-glycosylated integral membrane protein. Furthermore, BCMAp inserts, in vitro, into canine microsomes, as a type I integral membrane protein. Cell surface labeling showed that BCMAp is not expressed in the plasma membrane of mature B lymphocytes. Immunofluorescence studies revealed that BCMAp lies in a cap-like structure near the nucleus, that was identified as the Golgi apparatus by co-localization of BCMAp with CTR433, a marker of the medial cisternae of the Golgi apparatus. Confocal scanning laser microscopy of U266 plasma cells labeled with markers of various Golgi apparatus subcompartments strongly suggests that BCMAp is located in the cis part of the Golgi apparatus. Thus, BCMAp is the first Golgi resident protein with a tissue specificity and whose expression is linked to the stage of differentiation of B lymphocytes. The location of BCMAp in the Golgi apparatus and its high expression in plasmocytes (secreting large amounts of Ig) suggest that BCMAp is implicated in the intracellular traffic of Ig.