Evidence that the Saccharomyces cerevisiae CIF1 (GGS1/TPS1) gene modulates heat shock response positively

The CIFI gene (also called GGSIITPSI) encodes a protein of the trehalose synthase complex that affects trehalose accumulation and general glucose sensing by Saccharomyces cerevisiae cells. There is considerable debate as to whether CIFI-dependent trehalose accumulation is a determinant in heat shock...

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Bibliographic Details
Published in:FEBS letters 1995-12, Vol.377 (3), p.457-460
Main Authors: Hazell, Brian W., Nevalainen, Helena, Attfield, Paul V.
Format: Article
Language:English
Subjects:
CIF1 (GGSI/TPSI)
Gene Expression Regulation, Fungal
Genes, Fungal
Glucosyltransferases - biosynthesis
Glucosyltransferases - genetics
Heat shock
Heat-Shock Proteins - genetics
Heat-Shock Response - genetics
Hot Temperature
Mutation
Recombinant Fusion Proteins - biosynthesis
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Stress response
Thermotolerance
Transcription, Genetic
Trehalose
Trehalose - metabolism
Yeast ( Saccharomyces cerevisiae)
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Summary:The CIFI gene (also called GGSIITPSI) encodes a protein of the trehalose synthase complex that affects trehalose accumulation and general glucose sensing by Saccharomyces cerevisiae cells. There is considerable debate as to whether CIFI-dependent trehalose accumulation is a determinant in heat shock-acquired thermotolerance. Thermosensitivity of cif1 mutants could alternatively, or also, be related to gene expression-signalling defects in such strains. Because many signal-dependent factors are involved in stress protection and repair in yeast, we have compared the expression of various stress response and heat shock genes in ‘isogenic’ CIFI and cif1 strains growing exponentially in galactose medium. Transcription of CTT1, CIF1, HSP26, HSP82, HSP104, SSA4 and UBI4 was notably lower in the cifI mutant following heat shock. Moreover, a single copy of chromosomally integrated HSP104-lacZ fusion gave up to 5.5-fold more heat shock induction in the CIF1 strain compared to the cif1 mutant. We conclude that reduced heat shock-acquired thermotolerance in cif1-deletion mutants growing exponentially on galactose is more likely to result from a general reduction in expression of stress response and heat shock genes, than simply or solely through deficiency of trehalose accumulation. The possible role of CIF1 in modulating stress response is discussed.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)01392-X