Fertilization and pregnancies following intracytoplasmic injection of testicular spermatozoa

Intracytoplasmic injection (ICSI) with testicular sperm was performed in 16 couples. All men had ductal obstruction and failed previous attempts of epididymal sperm microaspiration. Testis tissue was obtained by excisional biopsies and incubated in HEPES buffered EBSS medium over 24 h at 37 degrees...

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Bibliographic Details
Published in:Journal of assisted reproduction and genetics 1995-10, Vol.12 (9), p.627-631
Main Authors: OBRUCA, A, MOCK, K, FEICHTINGER, W, LUNGLMAYR, G
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Biopsy
Birth control
Female
Fertilization - physiology
Fertilization in Vitro - methods
Fertilization in Vitro - standards
Gynecology. Andrology. Obstetrics
Humans
Infertility, Male - physiopathology
Infertility, Male - therapy
Male
Medical sciences
Microinjections
Micromanipulation
Middle Aged
Oocytes - cytology
Oocytes - physiology
Pregnancy - physiology
Pregnancy Outcome
Pregnancy Rate
Sperm Motility - physiology
Sperm-Ovum Interactions - physiology
Spermatozoa - cytology
Spermatozoa - physiology
Sterility. Assisted procreation
Testis - cytology
Testis - physiology
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Summary:Intracytoplasmic injection (ICSI) with testicular sperm was performed in 16 couples. All men had ductal obstruction and failed previous attempts of epididymal sperm microaspiration. Testis tissue was obtained by excisional biopsies and incubated in HEPES buffered EBSS medium over 24 h at 37 degrees C. Motile sperm (Grade 1 to 2) were recovered in 13 patients and fertilized a total of 62 oozytes. Four pregnancies were achieved. One healthy boy and two girls (twin pregnancy) were born. The ongoing pregnancies revealed no fetal abnormalities on ultrasound scanning.
ISSN:1058-0468
1573-7330
DOI:10.1007/BF02212587