Effect of essential fatty acid deficiency on G-proteins, cAMP-dependent protein kinase activity and mucin secretion in the rat submandibular salivary glands

Studies were conducted to determine whether β-adrenergic cell signalling is altered in submandibular salivary glands (SMSG) is essential fatty acid (EFA) deficiency. Three groups of rats were fed diets which were deficient in EFA (EFAD), marginally deficient in EFA (MEFAD) or contained sufficient am...

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Bibliographic Details
Published in:Cellular signalling 1995-11, Vol.7 (8), p.773-781
Main Authors: Alam, Syed Q., Abdel-Hakim, Selim M., Alam, Bassima S., Ibrahim, Ibrahim Y.
Format: Article
Language:English
Subjects:
Adenylyl Cyclases - metabolism
Animals
Body Weight - drug effects
Cyclic AMP - physiology
Cyclic AMP-Dependent Protein Kinases - metabolism
Dietary Fats - administration & dosage
EFA deficiency
Fatty Acids, Essential - deficiency
Fatty Acids, Essential - metabolism
G-proteins
GTP-Binding Proteins - metabolism
Male
Mucin secretion
Mucins - secretion
PKA activity
Rats
Rats, Sprague-Dawley
Receptors, Adrenergic, beta - physiology
Saliva - secretion
Salivary glands
Salivary Proteins and Peptides - secretion
Secretory Rate - drug effects
Signal Transduction
Submandibular Gland - metabolism
Submandibular Gland - secretion
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Summary:Studies were conducted to determine whether β-adrenergic cell signalling is altered in submandibular salivary glands (SMSG) is essential fatty acid (EFA) deficiency. Three groups of rats were fed diets which were deficient in EFA (EFAD), marginally deficient in EFA (MEFAD) or contained sufficient amount of EFA (Control). Rats were killed after 20 wk on diets, SMSG were dissected out and cyclic AMP-dependent protein kinase (PKA) activity was measured. The specific enzyme activities were higher in the homogenates and supernatant fractions of the gland from EFAD and MEFAD rats compared with the controls. The relative levels of guanine nucleotide-binding regulatory proteins (Gs and Gi) were also measured in the SMSG membranes of rats fed the 3 diets. The levels of Gs were significantly higher in the EFAD and MEFAD groups than in the controls. No significant differences were observed in the secretion of trichloroacetic acid-phosphotungstic acid (TCA-PTA) precipitable glycoproteins from the SMSG slices among the 3 dietary groups.
ISSN:0898-6568
1873-3913
DOI:10.1016/0898-6568(95)02005-5