Growth of Human-Human Hybridomas in Serum-Free Media Enhances Antibody Secretion

Human-human hybridomas derived from fusing lymph node lymphocytes with UC 729-6 were adapted to grow in commercially available serum-free medium and were compared with serum-supplemented [10% fetal bovine serum (FBS)] cultures. Over a 6-d period, no significant changes occurred in the growth of the...

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Bibliographic Details
Published in:In Vitro Cellular & Developmental Biology 1987-11, Vol.23 (11), p.745-749
Main Authors: Glassy, Mark C., Robert E. Peters, Alexander Mikhalev
Format: Article
Language:English
Subjects:
Animal cells
Animals
Antibodies
Antibodies, Monoclonal - secretion
B lymphocytes
Biological and medical sciences
Biotechnology
Blood Physiological Phenomena
Cattle
Cell cultures. Hybridization. Fusion
Cell growth
Cell hybridization, cell fusion, hybridomas
Cell lines
Culture Media - pharmacology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
Gels
Humans
hybridoma
Hybridomas
Hybridomas - cytology
Hybridomas - immunology
Hybridomas - secretion
Immunoglobulin G - secretion
Immunoglobulin M - secretion
Lymph Nodes - cytology
Lymphocytes
Methods. Procedures. Technologies
Molecular and cellular biology
Monoclonal antibodies
Proteins
Secretion
Ungulates
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Summary:Human-human hybridomas derived from fusing lymph node lymphocytes with UC 729-6 were adapted to grow in commercially available serum-free medium and were compared with serum-supplemented [10% fetal bovine serum (FBS)] cultures. Over a 6-d period, no significant changes occurred in the growth of the cells in 10% FBS or serum-free medium. In cultures supplemented with 10% FBS more total proteins were secreted than in serum-free cultures. However, there was an enhanced secretion of three- to four-fold of both immunoreactive human IgG and IgM under serum-free conditions compared to serum-supplemented conditions. Serum-free conditions may provide the appropriate milieu for the increased level of Ig secretion from human hybridomas derived from UC 729-6 in that there are no inhibitors that may be present in serum.
ISSN:0883-8364
0073-5655
2327-431X
1475-2689
DOI:10.1007/BF02623674