Changes in the expression of fibroblast growth factor receptors mark distinct stages of chondrogenesis in vitro and during chick limb skeletal patterning

Members of the fibroblast growth factor (FGF) family of growth factors are key regulators of limb skeletal patterning and growth. Abnormal expression of FGFs or mutations in their receptors (fgfrs) result in skeletal disorders. Here we show that changes in the expression of fgfrs are intrinsic prope...

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Bibliographic Details
Published in:Developmental dynamics 1995-12, Vol.204 (4), p.446-456
Main Authors: Szebenyi, Györgyi, Savage, Mary Pfann, Olwin, Bradley B., Fallon, John F.
Format: Article
Language:English
Subjects:
Alternative Splicing - genetics
Animals
Base Sequence
Cartilage
Cartilage - cytology
Cartilage - embryology
Cartilage - ultrastructure
Cell Differentiation - physiology
Cells, Cultured - physiology
Chick Embryo
DNA, Complementary - physiology
Extremities - embryology
FGF
FGF receptors
Fibroblast Growth Factors - physiology
Gene Expression Regulation, Developmental - physiology
In Situ Hybridization
Limb Buds - physiology
Limb development
Mesoderm - cytology
Mesoderm - physiology
Micromass cultures
Molecular Sequence Data
Receptors, Fibroblast Growth Factor - genetics
RNA, Messenger - analysis
Time Factors
Wings, Animal - embryology
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Summary:Members of the fibroblast growth factor (FGF) family of growth factors are key regulators of limb skeletal patterning and growth. Abnormal expression of FGFs or mutations in their receptors (fgfrs) result in skeletal disorders. Here we show that changes in the expression of fgfrs are intrinsic properties of differentiating cartilage. In mesenchymal micromass cultures differentiating into cartilage, as in ovo, fgfr 1 mRNA was found predominantly in undifferentiated, proliferating mesenchyme, fgfr 2 in precartilage cell aggregates, and fgfr 3 in differentiating cartilage nodules. Thus, our data suggest that switches in the expression of fgfr 1, 2, and 3 mRNAs are associated with phases of cartilage patterning both in vitro and in ovo, and mark distinct stages in the development of the limb skeleton. © 1995 wiley‐Liss, Inc.
ISSN:1058-8388
1097-0177
DOI:10.1002/aja.1002040410