Endothelin-stimulated monocyte supernatants enhance neutrophil superoxide production

Endothelin-1 (ET-1) is a vasoconstrictive peptide released by ischemic/injured endothelium which increases intracellular ionized calcium [Ca2+]i in vascular smooth muscle. Previous work from this lab has shown that ET-1 also increases human peripheral blood monocyte [Ca2+]i, and that 24 h incubation...

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Bibliographic Details
Published in:Shock (Augusta, Ga.) Ga.), 1994-03, Vol.1 (3), p.184-187
Main Authors: Huribal, M, Kumar, R, Cunningham, M E, Sumpio, B E, McMillen, M A
Format: Article
Language:English
Subjects:
Cytochrome c Group - analysis
Cytochrome c Group - metabolism
Endothelins - metabolism
Endothelins - pharmacology
Escherichia coli - chemistry
Humans
Interleukin-8 - pharmacology
Lipopolysaccharides - pharmacology
Monocytes - chemistry
Monocytes - drug effects
Monocytes - metabolism
Neutrophils - drug effects
Neutrophils - metabolism
Superoxides - metabolism
Time Factors
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Summary:Endothelin-1 (ET-1) is a vasoconstrictive peptide released by ischemic/injured endothelium which increases intracellular ionized calcium [Ca2+]i in vascular smooth muscle. Previous work from this lab has shown that ET-1 also increases human peripheral blood monocyte [Ca2+]i, and that 24 h incubation of monocytes with 10(-9) M ET-1 causes production of prostaglandin E2 and interleukin-6. In these studies, ET-1-stimulated monocyte supernatants were evaluated for their effect on neutrophil superoxide production. While ET-1 alone had no direct effect, incubation of neutrophils for 20 min in ET-1-stimulated monocyte supernatants resulted in a 10-fold increase in superoxide production over basal levels, 44% as much superoxide production as induced by peptide N-formyl-methionyl-leucyl-phenylalanine (N = 6, p < .001). Monocyte supernatants were analyzed for interleukin-8 (IL-8 or neutrophil activation protein) content by radioimmunoassay. ET-1-stimulation resulted in production of 54% as much IL-8 as lipopolysaccharide controls (N = 6, p < .001). While a number of monokines can activate neutrophils, IL-8 has been shown to be a potent neutrophil activator as well as a superoxide primer. Therefore, ET-1-treated monocytes probably upregulate neutrophil superoxide production via a mechanism which includes IL-8.
ISSN:1073-2322
DOI:10.1097/00024382-199403000-00005