Parallel induction of cecropin and lysozyme in larvae of the silkworm, Bombyx mori

Lysozyme activity in the hemolymph of Bombyx mori increased in parallel with cecropin activity after injection of the larvae with soluble peptidoglycan or UV-killed bacteria. The lysozyme and cecropin A genes were expressed in parallel in the fat body after injection of peptidoglycan as detected by...

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Bibliographic Details
Published in:Developmental and comparative immunology 1995-09, Vol.19 (5), p.357-363
Main Authors: Morishima, Isao, Horiba, Tomoki, Iketani, Masao, Nishioka, Eri, Yamano, Yoshiaki
Format: Article
Language:English
Subjects:
Animals
Antimicrobial Cationic Peptides
Blotting, Northern
Bombyx - immunology
Bombyx mori
Cecropin
Gene Expression
Hemolymph - immunology
Insect Hormones - biosynthesis
Insect Hormones - genetics
Insect immunity
Lysozyme
Muramidase - biosynthesis
Muramidase - genetics
Peptide Biosynthesis
Peptides - genetics
Peptidoglycan
Peptidoglycan - immunology
Silkworm
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Summary:Lysozyme activity in the hemolymph of Bombyx mori increased in parallel with cecropin activity after injection of the larvae with soluble peptidoglycan or UV-killed bacteria. The lysozyme and cecropin A genes were expressed in parallel in the fat body after injection of peptidoglycan as detected by northern blot hybridization. The elicitor specificity for lysozyme induction was identical to that for cecropin, suggesting a common mechanism for recognition of bacteria and following signal transduction introducing to the simultaneous synthesis of cecropin and lysozyme. Bacterial cells killed by UV-irradition were also effective as elicitor when added to the fat body culture, suggesting that phagocytosis of bacteria by hemocytes may not be an essential process for the induction of antibacterial protein synthesis in the silkworm.
ISSN:0145-305X
1879-0089
DOI:10.1016/0145-305X(95)00019-P