The DNase I generated disomal series is coherent to 16N Implications for coiling models of chromatin structure

The disomal series generated by the digestion of chromatin with DNase I has been followed to its highest orders using Klenow end‐labelling and field‐inversion gel electrophoresis to maximise the resolution of large DNA fragments. The series is coherent to the 16N level and as such is incompatible wi...

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Bibliographic Details
Published in:FEBS letters 1987-12, Vol.226 (1), p.88-90
Main Authors: Davis, S.J., Burgoyne, L.A.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Chickens
Chromatin
Chromatin - ultrastructure
Chromatin. Chromosome
Deoxyribonuclease I
Disomal series
DNA - blood
DNA - ultrastructure
DNase I
Erythrocytes - ultrastructure
Field-inversion gel electrophoresis
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
Molecular genetics
Nucleic Acid Conformation
Structural model
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Summary:The disomal series generated by the digestion of chromatin with DNase I has been followed to its highest orders using Klenow end‐labelling and field‐inversion gel electrophoresis to maximise the resolution of large DNA fragments. The series is coherent to the 16N level and as such is incompatible with the most structurally acceptable coiling models. We propose that this is evidence for the general unsuitability of coiling models and is support for the existence of simple “back‐to‐back” double‐stranded structures within chromatin.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)80556-2