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The DNase I generated disomal series is coherent to 16N Implications for coiling models of chromatin structure

The disomal series generated by the digestion of chromatin with DNase I has been followed to its highest orders using Klenow end‐labelling and field‐inversion gel electrophoresis to maximise the resolution of large DNA fragments. The series is coherent to the 16N level and as such is incompatible wi...

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Published in:	FEBS letters 1987-12, Vol.226 (1), p.88-90
Main Authors:	Davis, S.J., Burgoyne, L.A.
Format:	Article
Language:	English
Subjects:	Animals Biological and medical sciences Chickens Chromatin Chromatin - ultrastructure Chromatin. Chromosome Deoxyribonuclease I Disomal series DNA - blood DNA - ultrastructure DNase I Erythrocytes - ultrastructure Field-inversion gel electrophoresis Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Nucleic Acid Conformation Structural model
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container_title	FEBS letters
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creator	Davis, S.J. Burgoyne, L.A.
description	The disomal series generated by the digestion of chromatin with DNase I has been followed to its highest orders using Klenow end‐labelling and field‐inversion gel electrophoresis to maximise the resolution of large DNA fragments. The series is coherent to the 16N level and as such is incompatible with the most structurally acceptable coiling models. We propose that this is evidence for the general unsuitability of coiling models and is support for the existence of simple “back‐to‐back” double‐stranded structures within chromatin.
doi_str_mv	10.1016/0014-5793(87)80556-2
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Chromosome</topic><topic>Deoxyribonuclease I</topic><topic>Disomal series</topic><topic>DNA - blood</topic><topic>DNA - ultrastructure</topic><topic>DNase I</topic><topic>Erythrocytes - ultrastructure</topic><topic>Field-inversion gel electrophoresis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Nucleic Acid Conformation</topic><topic>Structural model</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Davis, S.J.</creatorcontrib><creatorcontrib>Burgoyne, L.A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Davis, S.J.</au><au>Burgoyne, L.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The DNase I generated disomal series is coherent to 16N Implications for coiling models of chromatin structure</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1987-12-21</date><risdate>1987</risdate><volume>226</volume><issue>1</issue><spage>88</spage><epage>90</epage><pages>88-90</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><coden>FEBLAL</coden><abstract>The disomal series generated by the digestion of chromatin with DNase I has been followed to its highest orders using Klenow end‐labelling and field‐inversion gel electrophoresis to maximise the resolution of large DNA fragments. 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source	ScienceDirect Journals
subjects	Animals Biological and medical sciences Chickens Chromatin Chromatin - ultrastructure Chromatin. Chromosome Deoxyribonuclease I Disomal series DNA - blood DNA - ultrastructure DNase I Erythrocytes - ultrastructure Field-inversion gel electrophoresis Fundamental and applied biological sciences. Psychology Molecular and cellular biology Molecular genetics Nucleic Acid Conformation Structural model
title	The DNase I generated disomal series is coherent to 16N Implications for coiling models of chromatin structure
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