High Cell Density Cultivation and High Recombinant Protein Production of Escherichia coli Strain Expressing Uricase

Uricase from Cellulomonas flavigena SK-4 is an industrially useful enzyme for commercial formulations of hair coloring. The uricase production by recombinant Escherichia coli strain with a high cell density cultivation technique was described. Of three kinds of media, synthetic media with the feedin...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1995, Vol.59 (12), p.2263-2267
Main Authors: Nakagawa, Satoshi, Oda, Hideki, Anazawa, Hideharu
Format: Article
Language:English
Subjects:
Actinomycetales - enzymology
Bacteriological Techniques
Biological and medical sciences
Biotechnology
Escherichia coli - enzymology
Escherichia coli - genetics
Escherichia coli - growth & development
Fundamental and applied biological sciences. Psychology
Fungal Proteins - biosynthesis
Fungal Proteins - genetics
Genetic engineering
Genetic technics
Glucose - pharmacology
Methods. Procedures. Technologies
Modification of gene expression level
Oxygen - pharmacology
Recombinant Proteins - biosynthesis
Recombinant Proteins - genetics
Urate Oxidase - biosynthesis
Urate Oxidase - genetics
Urate Oxidase - metabolism
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Summary:Uricase from Cellulomonas flavigena SK-4 is an industrially useful enzyme for commercial formulations of hair coloring. The uricase production by recombinant Escherichia coli strain with a high cell density cultivation technique was described. Of three kinds of media, synthetic media with the feeding of a high concentration of glucose solution were suitable for high cell density cultivation. As for feeding, both biomass concentration and uricase productivity were increased by about two (61.2 g dry cell weight (DCW)/liter) and three times (1037 U/ml broth), respectively, in 24 h by continuous supply. In the case of feeding by a DO-stat method, however, cell concentration was comparable to continuous glucose supply but uricase activity was reduced. By supplying pure oxygen to compensate for oxygen limitation during cultivation, the highest values of 77.4g DCW/liter and 1113 U/ml broth of the uricase activity were achieved with the total cultivation time of 15h.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.59.2263