Hydroxyl radical footprinting of the sequence-selective binding of netropsin and distamycin to DNA

Hydroxyl radicals, generated by allowing an iron (II)·EDTA complex to react with hydrogen peroxide, have been employed to cleave the 160-base pair tyrT DNA fragment in the presence and absence of the minor groove-binding antibiotics netropsin and distamycin A. The control DNA cleavage pattern is pra...

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Bibliographic Details
Published in:FEBS letters 1987-12, Vol.225 (1), p.195-200
Main Authors: Portugal, J., Waring, M.J.
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Antibiotic-DNA binding
Base Composition
Base Sequence
Binding Sites
Biological and medical sciences
Distamycin A
Distamycins - metabolism
DNA, Bacterial - metabolism
Dna, deoxyribonucleoproteins
Edetic Acid
Escherichia coli - genetics
Ferric Compounds
Fundamental and applied biological sciences. Psychology
Guanidines - metabolism
Hydroxides - metabolism
Hydroxyl Radical
Hydroxyl radical footprinting
Iron (II)·EDTA complex
Molecular Sequence Data
Netropsin
Netropsin - metabolism
Nucleic acids
Pyrroles - metabolism
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Summary:Hydroxyl radicals, generated by allowing an iron (II)·EDTA complex to react with hydrogen peroxide, have been employed to cleave the 160-base pair tyrT DNA fragment in the presence and absence of the minor groove-binding antibiotics netropsin and distamycin A. The control DNA cleavage pattern is practically independent of nucleotide sequence, which overcomes certain limitations of other footprinting techniques, so that additional information can be gained about the AT-rich sequence preference of the minor groove-binding ligands.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)81156-0