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Establishment of immunological probes to study human gonadotropin-releasing hormone receptors

Monoclonal antibodies were raised to a synthetic peptide corresponding to amino acids 1–29 of the human gonadotropin-releasing hormone (GnRH) receptor. One of the two antibodies was found to recognise GnRH receptors on human pituitary gonadotrophs as determined by immunohistochemistry and supported...

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Bibliographic Details
Published in:Molecular and cellular endocrinology 1995-10, Vol.114 (1), p.51-56
Main Authors: Karande, A.A., Rajeshwari, K., Schol, D.J., Hilgers, J.H.M.
Format: Article
Language:English
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Summary:Monoclonal antibodies were raised to a synthetic peptide corresponding to amino acids 1–29 of the human gonadotropin-releasing hormone (GnRH) receptor. One of the two antibodies was found to recognise GnRH receptors on human pituitary gonadotrophs as determined by immunohistochemistry and supported by Western blotting. The antibody also bound to T47D human breast carcinoma cell line as determined by flow cytometric analysis.
ISSN:0303-7207
1872-8057
DOI:10.1016/0303-7207(95)03641-J