Age-related changes of oxygen free radical production in the rat brain slice after hypoxia: on-line measurement using enhanced chemiluminescence

Superoxide radical production in brain slices of 12-, 24- and 60-day-old rats was measured online during and after 15 min of hypoxia with lucigenin enhanced chemiluminescence. We found a typical radical burst after reoxygenation which developed with aging and is almost nonexistent in the youngest an...

Full description

Saved in:
Bibliographic Details
Published in:Brain research 1995-12, Vol.703 (1), p.227-230
Main Authors: Schreiber, Stephan J., Megow, Dirk, Raupach, Annett, Victorov, Ilya V., Dirnagl, Ulrich
Format: Article
Language:English
Subjects:
Aging - metabolism
Analysis of Variance
Animals
Biological and medical sciences
Brain - growth & development
Brain - metabolism
Brain development
Development. Senescence. Regeneration. Transplantation
Free Radical Scavengers - metabolism
Free Radicals
Fundamental and applied biological sciences. Psychology
Hypoxia, Brain - metabolism
In Vitro Techniques
Ischemia model
Lucigenin
Luminescent Measurements
Male
Online Systems
Rats
Rats, Wistar
Reactive Oxygen Species - metabolism
Reoxygenation
Scavenger
Superoxides - metabolism
Vertebrates: nervous system and sense organs
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Superoxide radical production in brain slices of 12-, 24- and 60-day-old rats was measured online during and after 15 min of hypoxia with lucigenin enhanced chemiluminescence. We found a typical radical burst after reoxygenation which developed with aging and is almost nonexistent in the youngest and most prominent in the oldest group. This cannot be explained by a decreasing tissue superoxide dismutase (SOD) concentration in the brain with aging, since the concentration of the enzyme, determined in the same age groups, increased with age.
ISSN:0006-8993
1872-6240
DOI:10.1016/0006-8993(95)01188-9