Augmentation by thyroxine of human granulosa cell gonadotrophin-induced steroidogenesis

The objective of this study was to investigate the influence of thyroid hormone on gonadotrophin-induced oestradiol and progesterone secretion by human granulosa cells maintained in vitro. Granulosa cells were obtained by aspiration of pre-ovulatory follicles from women undergoing assisted reproduct...

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Bibliographic Details
Published in:Human reproduction (Oxford) 1995-11, Vol.10 (11), p.2845-2848
Main Authors: Wakim, Anthony N., Polizotto, Sandra L., Burholt, Dennis R.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Chorionic Gonadotropin - pharmacology
Estradiol - biosynthesis
Estradiol - metabolism
Female
Follicle Stimulating Hormone - pharmacology
Fundamental and applied biological sciences. Psychology
Granulosa Cells - drug effects
Granulosa Cells - metabolism
human granulosa cells
Humans
In Vitro Techniques
Infertility - metabolism
Infertility - therapy
Luteinizing Hormone - pharmacology
Mammalian female genital system
Menotropins - pharmacology
Morphology. Physiology
oestradiol secretion
Ovulation Induction
Progesterone - biosynthesis
Progesterone - metabolism
progesterone secretion
thyroid hormone
Thyroxine - pharmacology
Vertebrates: reproduction
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Summary:The objective of this study was to investigate the influence of thyroid hormone on gonadotrophin-induced oestradiol and progesterone secretion by human granulosa cells maintained in vitro. Granulosa cells were obtained by aspiration of pre-ovulatory follicles from women undergoing assisted reproductive technology. Ovulation induction was performed with gonadotrophin-releasing hormone agonist, human menopausal gonadotrophin and human chorionic gonadotrophin. Granulosa cells were maintained in vitro in a defined medium with added insulin. Between 48 and 72 h after the initiation of cell culture, oestradiol and progesterone secretion into the medium was determined for granulosa cells growing in serum-free medium with follicle-stimulating hormone (FSH)/luteinizing hormone (LH) and in serum-free medium with FSH/LH and thyroxine added in a concentration range of 10−10–10−7 M. All concentrations of thyroxine used produced a statistically significant increase in oestradiol (range 1.18−1.37 times the amount with FSH/LH alone) and progesterone (range 1.29−1.51 times the amount with FSH/LH alone) secretion.
ISSN:0268-1161
1460-2350
DOI:10.1093/oxfordjournals.humrep.a135805