Localization of mRNA Coding for the Three Subtypes of Atrial Natriuretic Factor (ANF) Receptors in Rat Anterior Pituitary Gland Cells

Atrial Natriuretic Factor (ANF) action is mediated by highly selective and specific receptors. Three subtypes have been characterized and cloned: ANF receptor‐A, ‐B and ‐C. These subtypes are all expressed in the anterior pituitary of the rat. In the present study, the mRNA for each subtype was dete...

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Bibliographic Details
Published in:Journal of neuroendocrinology 1995-12, Vol.7 (12), p.939-948
Main Authors: Grandclément, Béatrice, Brisson, Christine, Bayard, Francis, Tremblay, Johanne, Gossard, Francis, Morel, Gerard
Format: Article
Language:English
Subjects:
ANFR-A
ANFR-B
ANFR-C
Animals
anterior pituitary
Atrial Natriuretic Factor
Base Sequence
Cloning, Molecular
Cytoplasmic Granules - metabolism
Cytoplasmic Granules - ultrastructure
electron microscopy
Endoplasmic Reticulum - metabolism
Endoplasmic Reticulum - ultrastructure
Female
Gene Expression
Growth Hormone - biosynthesis
Guanylate Cyclase - analysis
Guanylate Cyclase - biosynthesis
In Situ Hybridization
Male
Microscopy, Electron
Molecular Sequence Data
mRNA
Oligonucleotide Probes
Pituitary Gland, Anterior - cytology
Pituitary Gland, Anterior - metabolism
Rats
Rats, Wistar
Receptors, Atrial Natriuretic Factor - analysis
Receptors, Atrial Natriuretic Factor - biosynthesis
Recombinant Proteins - biosynthesis
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
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Summary:Atrial Natriuretic Factor (ANF) action is mediated by highly selective and specific receptors. Three subtypes have been characterized and cloned: ANF receptor‐A, ‐B and ‐C. These subtypes are all expressed in the anterior pituitary of the rat. In the present study, the mRNA for each subtype was detected by in situ hybridization. The amounts of ANFR‐A and ‐B mRNA were found to be similar, and to be twice that of ANFR‐C mRNA. At the ultrastructural level, the three types of ANFR mRNA were expressed in three anterior pituitary cell types, namely lactotrophs, corticotrophs, and gonadotrophs, identified by their hormonal content. No signal was revealed in somatotrophs or thyrotrophs. The different forms of mRNA were similar in terms of subcellular localization: in the cytoplasmic matrix and the nuclear euchromatin. These data indicate that the anterior pituitary is an important target tissue for ANF action.
ISSN:0953-8194
1365-2826
DOI:10.1111/j.1365-2826.1995.tb00739.x