Type XII collagen is expressed in embryonic chick tendons. Isolation of pepsin-derived fragments

Two new disulfide-bonded collagenous pepsin-derived fragments have been isolated from chick embryo tendons. These fragments represent less than 0.5% of the pepsin-extractable collagen. We purified 10- and 16-kDa reduced fragments derived, respectively, from 32- and 46-kDa disulfide-bonded fragments....

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Bibliographic Details
Published in:The Journal of biological chemistry 1987-12, Vol.262 (36), p.17724-17727
Main Authors: Dublet, B, van der Rest, M
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological and medical sciences
Biotechnology
Cell coat. Cell surface
Cell structures and functions
Chick Embryo
Chromatography, High Pressure Liquid
Collagen - biosynthesis
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Methods. Procedures. Technologies
Molecular and cellular biology
Molecular Weight
Pepsin A - metabolism
Peptide Fragments - isolation & purification
Tendons - embryology
Tendons - metabolism
Trypsin - metabolism
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Summary:Two new disulfide-bonded collagenous pepsin-derived fragments have been isolated from chick embryo tendons. These fragments represent less than 0.5% of the pepsin-extractable collagen. We purified 10- and 16-kDa reduced fragments derived, respectively, from 32- and 46-kDa disulfide-bonded fragments. Unique tryptic peptide maps were obtained for each fragment. Although these collagenous fragments differ from all the known collagens in their electrophoretic behaviors, tryptic peptides and amino acid compositions, they present some striking similarities with the pepsin-derived fragments from cartilage type IX collagen. Unique sequences from the amino terminus and from a tryptic peptide were obtained for the 10-kDa reduced fragment, demonstrating that this fragment is derived from type XII collagen, whose existence had only been inferred so far from a cDNA encoding it.
ISSN:0021-9258
1083-351X
DOI:10.1016/S0021-9258(18)45439-7