Regulation of Pituitary Vasopressin V1b Receptor mRNA during Stress in the Rat

Previous studies have shown a parallel relationship between pituitary vasopressin (VP) receptor content and responsiveness of the corticotroph during chronic stress. The regulation of pituitary VP receptors was further studied by analysis of V1b VP receptor mRNA levels in pituitaries of rats subject...

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Bibliographic Details
Published in:Journal of neuroendocrinology 1995-12, Vol.7 (12), p.903-910
Main Authors: Rabadan-Diehl, Cristina, Lolait, Stephen J., Aguilera, Greti
Format: Article
Language:English
Subjects:
Animals
Antisense Elements (Genetics)
Blotting, Northern
Cell Membrane - metabolism
Gene Expression Regulation
In Situ Hybridization
Inositol Phosphates - metabolism
Male
mRNA levels
pituitary corticotroph
Pituitary Gland - metabolism
Rats
Rats, Sprague-Dawley
Receptors, Vasopressin - biosynthesis
Receptors, Vasopressin - metabolism
Restraint, Physical
Restriction Mapping
RNA Probes
RNA, Messenger - analysis
RNA, Messenger - biosynthesis
Saline Solution, Hypertonic - administration & dosage
Saline Solution, Hypertonic - pharmacology
Space life sciences
stress
Stress, Physiological
Stress, Psychological
V1b receptor
vasopressin receptor
Vasopressins - metabolism
Water Deprivation
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Summary:Previous studies have shown a parallel relationship between pituitary vasopressin (VP) receptor content and responsiveness of the corticotroph during chronic stress. The regulation of pituitary VP receptors was further studied by analysis of V1b VP receptor mRNA levels in pituitaries of rats subjected to chronic immobilization, i.p. hypertonic saline injection (physical stress paradigms associated with increased pituitary responsiveness), and water deprivation, or to 2% saline in the drinking water (osmotic stress paradigms associated with decreased pituitary responsiveness). Northern blot hybridization with a 363 bp 32P‐labelled fragment of the rV1b receptor cDNA coding sequence revealed two bands of about 3.7 and 3.2 Kb, whereas a probe directed to the 5′ untranslated region recognized only the 3.7 Kb band. Repeated i.p. hypertonic saline injection, 3 times in 24 h at 8 h intervals, or daily for 8 days, increased the intensity of the 3.7 Kb band by 155 ± 17.5% (P
ISSN:0953-8194
1365-2826
DOI:10.1111/j.1365-2826.1995.tb00734.x