Identification of Base Pairs in the outside End of Insertion Sequence IS50 that are Needed for IS50 and Tn5 Transposition

Short DNA sequences at ends of transposable elements are needed as sites for transposition. Previous deletion mapping showed that, in Tn5 and its component IS50 elements, these essential sites are about 19 base pairs long. To determine which positions are important in transposition, we made one or m...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1987-12, Vol.84 (24), p.9118-9122
Main Authors: Phadnis, Suhas H., Berg, Douglas E.
Format: Article
Language:English
Subjects:
Bacterial Proteins - physiology
Bacteriophages
Base Sequence
Binding Sites
Biological and medical sciences
DNA
DNA Mutational Analysis
DNA Transposable Elements
Escherichia coli - genetics
Fundamental and applied biological sciences. Psychology
Genetic mutation
Genetic transposition
Genic rearrangement. Recombination. Transposable element
Infections
Molecular and cellular biology
Molecular genetics
Mutagenesis
Oligonucleotides
Plasmids
Recombination, Genetic
Structure-Activity Relationship
Transposons
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Description
Summary:Short DNA sequences at ends of transposable elements are needed as sites for transposition. Previous deletion mapping showed that, in Tn5 and its component IS50 elements, these essential sites are about 19 base pairs long. To determine which positions are important in transposition, we made one or more sequence changes at each position in the IS50 outside (O) end and assayed the effects of these changes on transposition. Our results indicate that the specific base pairs at 18 of the 19 positions are important in transposition. A 9-base-pair segment in the O end corresponds to a binding site for the Escherichia coli DnaA protein. Comparisons of effects of mutations at different positions in this site, and also measurements of Tn5 transposition in dnaA- cells, indicate that DnaA protein participates in O-end-mediated transposition.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.84.24.9118