Generation of OH radical during enzymatic reduction of 9,10-anthraquinone-2-sulphonate Can semiquinone decompose hydrogen peroxide?

Spin-trapping experiments have shown that 9, 10-anthraquinone-2-sulphonate (AQS) can be reduced enzymatically with NADPH-cytochrome P-450 reductase (FP) producing OH radicals. It has been revealed for the first time that the radical anion of AQS cannot decompose H 2O 2 as indicated by flash-photolys...

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Bibliographic Details
Published in:FEBS letters 1987-12, Vol.225 (1), p.139-144
Main Authors: Sushkov, D.G., Gritsan, N.P., Weiner, L.M.
Format: Article
Language:English
Subjects:
Anthraquinones - metabolism
Antineoplastic agents
AQS, 9,10-anthraquinone-2-sulphonate (Na salt)
Benzoquinones
Biological and medical sciences
Cyclic N-Oxides
DMPO, 5,5-dimethylpyrroline N-oxide
DMSO, dimethyl sulphoxide
Electron Spin Resonance Spectroscopy
FP, NADPH-cytochrome P-450 reductase
General aspects
Hydrogen peroxide
Hydrogen Peroxide - metabolism
Hydroxides - metabolism
Hydroxyl Radical
Kinetics
Medical sciences
NADP - metabolism
NADPH-cytochrome P-450 reductase
NADPH-Ferrihemoprotein Reductase - metabolism
Oxidation-Reduction
Oxygen - metabolism
Pharmacology. Drug treatments
Photolysis
Quinone
Quinones - metabolism
Reaction mechanism
SOD, superoxide dismutase
Spin Labels
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Summary:Spin-trapping experiments have shown that 9, 10-anthraquinone-2-sulphonate (AQS) can be reduced enzymatically with NADPH-cytochrome P-450 reductase (FP) producing OH radicals. It has been revealed for the first time that the radical anion of AQS cannot decompose H 2O 2 as indicated by flash-photolysis data. From the experimental results obtained it follows that an enzyme system containing NADPH, AQS and FP produces both accumulation of H 2O 2 from molecular oxygen and reduction of Fe 3+ to Fe 2+ which decomposes H 2O 2 catalytically via a Fenton reaction.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(87)81146-8