Avian mycoplasma identification using polymerase chain reaction amplicon and restriction fragment length polymorphism analysis

A general mycoplasma polymerase chain reaction (PCR) was used to generate amplicon (DNA amplification product) from nine avian mycoplasma species. The PCR amplicons were reacted with 24 restriction enzymes, and the electrophoretic patterns of restriction fragment length polymorphism (RFLP) were eval...

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Bibliographic Details
Published in:Avian diseases 1995-10, Vol.39 (4), p.804-811
Main Authors: Lauerman, L.H. (Alabama Department of Agriculture and Industries, Auburn, AL.), Chilina, A.R, Closser, J.A, Johansen, D
Format: Article
Language:English
Subjects:
AMPLIFICATION CHAINE POLYMERASE
Animals
Base Sequence
Biochemistry
Chickens
Deoxyribonucleases, Type II Site-Specific
DIAGNOSTIC
DIAGNOSTICO
DIFERENCIAS BIOLOGICAS
DIFFERENCE BIOLOGIQUE
DNA
DNA Primers
DNA, Bacterial - analysis
Electrophoresis, Agar Gel
Enzymes
Gels
IDENTIFICACION
IDENTIFICATION
Molecular biology
Molecular Sequence Data
MYCOPLASMA
Mycoplasma - classification
Mycoplasma - genetics
Mycoplasma - isolation & purification
Mycoplasma gallisepticum
Mycoplasma Infections - diagnosis
Mycoplasma Infections - veterinary
Polymerase chain reaction
Polymerase Chain Reaction - methods
Polymerase Chain Reaction - veterinary
Polymorphism, Restriction Fragment Length
Poultry Diseases
REACCION DE CADENAS DE POLIMERASA
Restriction fragment length polymorphism
Restriction Mapping
RFLP
rRNA genes
Species Specificity
Turkeys
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Description
Summary:A general mycoplasma polymerase chain reaction (PCR) was used to generate amplicon (DNA amplification product) from nine avian mycoplasma species. The PCR amplicons were reacted with 24 restriction enzymes, and the electrophoretic patterns of restriction fragment length polymorphism (RFLP) were evaluated for differences between species of mycoplasmas. Four (DraI, MseI, RsaI, Tsp509I) of the 24 restriction enzymes cut the PCR amplicon of all nine mycoplasma species. The nine avian mycoplasma species could be distinctly differentiated using the RFLP analysis of the PCR amplicon
ISSN:0005-2086
1938-4351
DOI:10.2307/1592417