Reason for non-aphid transmissibility in a strain of Kalanchoe mosaic potyvirus

Kalanchoë mosaic potyvirus (KMV) is transmitted by aphids in a nonpersistent manner. After multiple mechanical inoculations, a non-aphid-transmissible (NAT) variant appeared. Addition of purified helper component (HC) from potato virus Y-infected plants did not restore the aphid transmissibility of...

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Bibliographic Details
Published in:Virus genes 1995-01, Vol.11 (1), p.59-61
Main Author: Husted, K. (Danish Institute of Plant and Soil Science, Lyngby, Denmark.)
Format: Article
Language:English
Subjects:
Amino Acid Sequence
AMINO ACID SEQUENCES
Animals
Aphids - virology
Base Sequence
Capsid - genetics
CHEMICAL COMPOSITION
COAT PROTEINS
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
DISEASE TRANSMISSION
DISEASE VECTORS
DNA, Viral - genetics
GENE
GENES
KALANCHOE BLOSSFELDIANA
Molecular Sequence Data
MUTACION
MUTATION
MYZUS PERSICAE
NUCLEOTIDE SEQUENCE
Oligopeptides - genetics
Plants - virology
POTYVIRUS
Potyvirus - genetics
Potyvirus - pathogenicity
POTYVIRUSES
PROTEINAS
PROTEINE
PROTEINS
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
STRUCTURAL GENES
TRANSMISION DE ENFERMEDADES
TRANSMISSION DES MALADIES
VECTEUR DE MALADIE
VECTORES
VECTORS
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Summary:Kalanchoë mosaic potyvirus (KMV) is transmitted by aphids in a nonpersistent manner. After multiple mechanical inoculations, a non-aphid-transmissible (NAT) variant appeared. Addition of purified helper component (HC) from potato virus Y-infected plants did not restore the aphid transmissibility of purified KMV-NAT. Comparison of the nucleotide sequence in the N-terminal half of the coat protein gene of KMV-NAT with that of an aphid-transmissible (AT) KMV strain revealed a single nucleotide difference (G to A). This nucleotide change turns a glycine residue (G) in the KMV-AT isolate into a glutamic acid residue (E) in the KMV-NAT isolate, abolishing the Asp-Ala-Gly (DAG) sequence, which has previously been shown to be essential for aphid transmission. The experiments show that non-aphid transmissibility is most likely caused by the change in the DAG sequence.
ISSN:0920-8569
1572-994X
DOI:10.1007/BF01701663