Cellular localisation and co-expression of somatostatin receptor messenger RNAs in the human brain

• Published in: Brain research. Molecular brain research. 1995-12, Vol.34 (2), p.321-326
• Main Authors: Schindler, Marcus, Harrington, Kathleen A., Humphrey, Patrick P.A., Emson, Piers C.
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; Biological and medical sciences; Central nervous system; Central neurotransmission. Neuromudulation. Pathways and receptors; Cerebellum - chemistry; Digoxigenin; Female; Frontal Lobe - chemistry; Fundamental and applied biological sciences. Psychology; G-protein coupled receptors; Hippocampus - chemistry; Humans; In situ hybridisation; In Situ Hybridization; Male; Middle Aged; Receptors, Somatostatin - genetics; Riboprobes; RNA, Messenger - analysis; RT-PCR; Vertebrates: nervous system and sense organs
• ISSN: 0169-328X; 1872-6941
• DOI: 10.1016/0169-328X(95)00191-T

Genes for five high affinity somatostatin receptors, named sst 1–5, have been cloned recently. In this study we describe the tissue distribution and cellular localisation of mRNA encoding sst 1, sst 3 and sst 4 receptors in the human cerebellum, frontal cortex (Brodmann's area 11) and hippocampus. RT-PCR and in situ hybridisation studies indicated a distinct, but partially overlapping pattern of expression of the receptor mRNAs. In situ hybridisation studies using co-expression techniques with probes for sst 1, sst 3 and sst 4 receptor mRNA on paraffin sections revealed the presence of neurones expressing more than one somatostatin receptor mRNA type in both the hippocampus and pyramidal cells of layer V of the frontal cortex.