Effects of Ca2+ and calmodulin on adenylyl cyclase activity in sheep olfactory epithelium

Sheep olfactory epithelium contains an adenylyl cyclase which is stimulated by many but not all odorants. Here we report that this enzyme is activated by calmodulin in a dose-dependent manner, and that calcium ions are required for this response. Odorant stimulation of adenylyl cyclase is unaffected...

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Bibliographic Details
Published in:Neurochemical research 1995-12, Vol.20 (12), p.1511-1517
Main Authors: FABBRI, E, FERRETTI, M. E, BUZZI, M, COLAMUSSI, M. L, BIONDI, C
Format: Article
Language:English
Subjects:
3',5'-Cyclic-AMP Phosphodiesterases - metabolism
Adenylyl Cyclases - metabolism
Animals
Biological and medical sciences
Calcium - pharmacology
Calmodulin - pharmacology
Enzyme Activation - drug effects
Epithelium - enzymology
Female
Fundamental and applied biological sciences. Psychology
Guanosine Triphosphate - pharmacology
Guanylyl Imidodiphosphate - pharmacology
Male
Odorants
Olfactory Mucosa - enzymology
Olfactory system and olfaction. Gustatory system and gustation
Sheep
Vertebrates: nervous system and sense organs
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Summary:Sheep olfactory epithelium contains an adenylyl cyclase which is stimulated by many but not all odorants. Here we report that this enzyme is activated by calmodulin in a dose-dependent manner, and that calcium ions are required for this response. Odorant stimulation of adenylyl cyclase is unaffected by the complex Ca2+/calmodulin, as suggested by the results obtained both in Ca2+/calmodulin-depleted membranes and under calmodulin antagonist treatment; this confirms the prediction that the Ca2+ binding protein and odorants stimulate the olfactory adenylyl cyclase through parallel mechanisms. The persistent activation of the regulatory component of adenylyl cyclase by GppNHp does not alter the response of the enzyme to either odorant or Ca2+/calmodulin. In sheep olfactory epithelium a cAMP-phosphodiesterase activity is also present, which is highly inhibited by IBMX and aminophylline, scarcely by RO 20-1724, and unaffected by Ca2+/calmodulin. The modulatory role exerted by calcium on cAMP system in sheep olfactory signal transduction is discussed.
ISSN:0364-3190
1573-6903
DOI:10.1007/BF00970601