The CDC6 gene is required for centromeric, episomal, and 2-microns plasmid stability in the yeast Saccharomyces cerevisiae

Temperature-sensitive Saccharomyces cerevisiae cdc6 mutants, under restrictive conditions, show an increase in recombination frequency, as well as chromosome and circular minichromosome loss. The role of the essential CDC6 gene was tested in trans and in cis to study circular plasmid stability. It w...

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Bibliographic Details
Published in:Plasmid 1995-11, Vol.34 (3), p.184-197
Main Authors: Storici, F, Oberto, J, Bruschi, C V
Format: Article
Language:English
Subjects:
Cell Cycle Proteins - genetics
Centromere
Gene Expression Regulation, Fungal - genetics
Genes, Fungal - genetics
Mutation
Plasmids
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae Proteins
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Summary:Temperature-sensitive Saccharomyces cerevisiae cdc6 mutants, under restrictive conditions, show an increase in recombination frequency, as well as chromosome and circular minichromosome loss. The role of the essential CDC6 gene was tested in trans and in cis to study circular plasmid stability. It was possible to demonstrate that the product of the CDC6 gene, acting in trans, is important for centromeric, episomal, and also 2-microns plasmid maintenance, while the gene sequence itself has no effect in cis on the stability of the plasmids tested. A high percentage of phenotypic revertants for the cdc6 mutation loses 2 microns upon shifting to the restrictive temperature and, under semipermissive conditions, the endogenous plasmid becomes very unstable, favoring a more efficient curing procedure. A positive correlation between centromeric plasmid size and stability was demonstrated even for small circular plasmids.
ISSN:0147-619X