Characterization and replication mode determination of the minimal replicon of Tetragenococcus halophila ATCC33315 plasmid pUCL287

pUCL287 is a cryptic plasmid of Tetragenococcus halophila (formerly Pediococcus halophilus) ATCC33315 of relatively small size (8.7 kb). Its minimal replicon was located on a 1235 bp MamI- EcoRI fragment. This minimal replicon contains a non-translated region, followed by a gene encoding a putative...

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Bibliographic Details
Published in:Biochimie 1995, Vol.77 (11), p.868-874
Main Authors: Benachour, A., Frère, J., Boutibonnes, P., Auffray, Y.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Base Sequence
DNA, Bacterial - genetics
Enterococcus faecalis
Molecular Sequence Data
Pediococcus
Pediococcus - genetics
plasmid
Plasmids - genetics
Replication Origin - genetics
Replicon - genetics
Tetragenococcus
Tetragenococcus halophila
theta replication
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Summary:pUCL287 is a cryptic plasmid of Tetragenococcus halophila (formerly Pediococcus halophilus) ATCC33315 of relatively small size (8.7 kb). Its minimal replicon was located on a 1235 bp MamI- EcoRI fragment. This minimal replicon contains a non-translated region, followed by a gene encoding a putative 311 amino acid protein. Deletion experiments showed that the non-translated region corresponds to the replication origin. Determination of the replication mode was carried out in Enterococcus faecalis JH2-2 harboring pUCL287 minimal replication. The replicating detected revealed that pUCL287 minimal replicon follows a bidirectional theta replicating mode.
ISSN:0300-9084
1638-6183
DOI:10.1016/0300-9084(95)90005-5