Re-evaluation of anti-HTLV-I Western blot assay using HTLV-I and HTLV-II serum panels

Background: Western blot assay is accepted to be a confirmatory test of anti-HTLV-I antibodies, and several WB criteria have been proposed for confirming HTLV-I seropositivity. There is a necessity for comprehensive study on the criteria. Objectives: This study was performed to evaluate anti-HTLV-I...

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Bibliographic Details
Published in:Clinical and diagnostic virology 1995-08, Vol.4 (2), p.149-161
Main Authors: Fujiyama, Chihaya, Fujiyoshi, Toshinobu, Matsumoto, Daiten, Yashiki, Shinji, Tamashiro, Hidehiko, Sonoda, Shunro
Format: Article
Language:English
Subjects:
HTLV-I
HTLV-II
Western blot criterion
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Summary:Background: Western blot assay is accepted to be a confirmatory test of anti-HTLV-I antibodies, and several WB criteria have been proposed for confirming HTLV-I seropositivity. There is a necessity for comprehensive study on the criteria. Objectives: This study was performed to evaluate anti-HTLV-I WB kits and feasibility of the WB criteria. Study design: We tested 3 commercially available WB kits: PROBLOT HTLV-I, Eitest ATL-WB and HTLV BLOT 2.2, by a standard HTLV-I serum panel which had been established in our previous study. Sensitivity and specificity to detect each component of HTLV-I antigens were evaluated by the correlation coefficient, R-value. Specificity of the anti-HTLV-I WB kits and HTLV BLOT 2.3 was further evaluated by a standard HTLV-II serum panel established from the HTLV-I II epidemiological surveillance of Colombian natives. Results: PROBLOT HTLV-I showed high R-values (⩾0.980) for p28, p53 and gp46, but low R-values (
ISSN:0928-0197
1873-4901
DOI:10.1016/0928-0197(95)00008-V