Assessment of the fertilizing potential of frozen bovine spermatozoa by in vitro induction of acrosome reactions with calcium ionophore (A23187)

The relationship between nonreturn rates of bulls in a commercial artificial insemination program and in vitro induction of acrosome reactions in frozen-thawed spermatozoa by the calcium ionophore, A23187, was investigated. Washed spermatozoa from 3 to 5 ejaculates, collected from each of 23 Holstei...

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Bibliographic Details
Published in:Theriogenology 1995-08, Vol.44 (3), p.413-422
Main Authors: Whitfield, C.H., Parkinson, T.J.
Format: Article
Language:English
Subjects:
ACROSOMA
ACROSOME
acrosome reaction
BOVIN LAITIER
bull
CALCIO
CALCIUM
ESPERMATOZOO
FECONDATION IN VITRO
FECUNDACION IN VITRO
FERTILIDAD
FERTILITE
fertility
GANADO DE LECHE
IONOFOROS
IONOPHORE
MACHO
MALE
MOUVEMENT
MOVIMIENTO
PERFORMANCE DE REPRODUCTION
REPRODUCTIVIDAD
semen
SPERMATOZOIDE
TAUREAU
TORO
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Summary:The relationship between nonreturn rates of bulls in a commercial artificial insemination program and in vitro induction of acrosome reactions in frozen-thawed spermatozoa by the calcium ionophore, A23187, was investigated. Washed spermatozoa from 3 to 5 ejaculates, collected from each of 23 Holstein bulls, were incubated for 1 h with 1 μM A23187. Acrosome reactions were determined by fluorescence microscopy. The percentage of increase in acrosome reaction in the ionophore-treated compared with control samples was significantly correlated to the 90-d nonreturn rate of the bulls (r = 0.86; P < 0.001). In a second experiment, a significant correlation was obtained between the fertility of bulls predicted on the basis of induced acrosome reaction and achieved 90-d nonreturn rate (r = 0.84; P < 0.005). No other assessments of semen quality (post-freezing motility, percentage of morphologically normal spermatozoa) was significantly correlated with fertility. Finally, the regression between acrosome reaction induction obtained from young bulls was used to predict the fertility of mature bulls whose semen was in widespread use (actual versus predicted nonreturn rate, r = 0.88; P < 0.0001).
ISSN:0093-691X
1879-3231
DOI:10.1016/0093-691X(95)00195-E