Promoter and expression studies on an Arabidopsis thaliana dehydrin gene

• Published in: FEBS letters 1996-03, Vol.381 (3), p.252-256
• Main Authors: Rouse, Dean T., Marotta, Rosetta, Parish, Roger W.
• Format: Article
• Language: English
• Subjects: Abscisic acid; Abscisic Acid - pharmacology; Amino Acid Sequence; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis thaliana; Base Sequence; Chromosome Mapping; Cloning, Molecular; Dehydrins; Gene Expression - drug effects; Genes, Plant; Glucuronidase - biosynthesis; Molecular Sequence Data; Plant Leaves; Plant Proteins - biosynthesis; Plant Proteins - genetics; Plant Roots; Plant Stems; Pollen; Promoter; Promoter Regions, Genetic; Recombinant Fusion Proteins - biosynthesis; Stress; TATA Box; Tissue expression; Wounds and Injuries
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(96)00051-8

A genomic clone of a group 2 lealrabldehydrin gene from Arabidopsis thaliana, Xero2llti30, was cloned and sequenced. Promoter- GUS fusions were introduced into plants to analyse the promoter and determine expression patterns. Using root cultures, GUS expression was found to be moderately stimulated by abscisic acid (ABA), wounding, cold and dehydration. Results with an ABA-deficient mutant suggested endogenous ABA is required for these responses. Promoter deletion studies indicated multiple cis-acting elements are involved in the induction of the gene. GUS expression occurred in desiccated seeds, in all tissues of young seedlings and in roots (with the exception of the root tip), desiccated pollen grains, trichomes and the vascular tissues of leaves and stems in mature plants.