Characterization of a cDNA encoding cysteine proteinase inhibitor from Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower buds

A cDNA encoding a new phytocystatin isotype named BCPI-1 was isolated from a cDNA library of Chinese cabbage flower buds. The BCPI-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. BCPI-1 has an unusually long C-terminus. A BCPI-1 fusion protein expr...

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Bibliographic Details
Published in:Plant molecular biology 1996-01, Vol.30 (2), p.373-379
Main Authors: Lim, C.O. (Gyeongsang National Univ., Chinju (Korea Republic). Dept. of Biochemistry), Lee, S.I, Chung, W.S, Park, S.H, Hwang, I, Cho, M.J
Format: Article
Language:English
Subjects:
ADN
Amino Acid Sequence
Base Sequence
Brassica - genetics
BRASSICA CAMPESTRIS
CISTEINA
CODE GENETIQUE
CODIGO GENETICO
CYSTEINE
Cysteine Proteinase Inhibitors - biosynthesis
Cysteine Proteinase Inhibitors - genetics
Cysteine Proteinase Inhibitors - pharmacology
DNA
DNA, Complementary - genetics
Escherichia coli - genetics
EXPRESION GENICA
EXPRESSION DES GENES
Gene Dosage
GENE EXPRESSION
Genes, Plant
GENETIC CODE
Molecular Sequence Data
Multigene Family
Papain - antagonists & inhibitors
Plant Proteins - biosynthesis
Plant Proteins - genetics
Plant Proteins - pharmacology
Plant Shoots
Recombinant Fusion Proteins - biosynthesis
Recombinant Fusion Proteins - pharmacology
Sequence Analysis, DNA
Sequence Homology, Amino Acid
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Summary:A cDNA encoding a new phytocystatin isotype named BCPI-1 was isolated from a cDNA library of Chinese cabbage flower buds. The BCPI-1 clone encodes 199 amino acids resulting in a protein much larger than other known phytocystatins. BCPI-1 has an unusually long C-terminus. A BCPI-1 fusion protein expressed in Escherichia coli strongly inhibits the enzymatic activity of papain, a cysteine proteinase. Genomic Southern blot analysis revealed that the BCPI gene is a member of a small multi-gene family in Chinese cabbage. Northern blot analysis showed that it is differentially expressed in the flower bud, leaf and root.
ISSN:0167-4412
1573-5028
DOI:10.1007/bf00020124