In situ localization of nucleolin in the plant nucleolar matrix

The analysis of isolated nucleolar matrices from onion cells by light and electron microscopy, 2-D separation of proteins, and confocal microscopy has confirmed the existence of an organized nucleolar matrix with a complex protein composition to which are attached the insoluble processing complexes....

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Bibliographic Details
Published in:Experimental cell research 1996-01, Vol.222 (1), p.171-178
Main Authors: Minguez, A. (Laboratorio de Biologia Celular y Molecular Vegetal, Centro de Investigaciones Biologicas, Madrid, Spain.), Moreno Diaz de la Espina, S
Format: Article
Language:English
Subjects:
Allium - chemistry
Allium - ultrastructure
ALLIUM CEPA
Bismuth
Cell Nucleolus - chemistry
CELLS
CELLULE
CELULAS
Coloring Agents
IMMUNOCYTOCHEMISTRY
IMMUNOFLUORESCENCE
IMMUNOLOGIE
IMMUNOLOGY
INMUNOFLUORESCENCIA
INMUNOLOGIA
MERISTEMAS
MERISTEME
MERISTEMS
Molecular Weight
Nuclear Matrix - chemistry
Nuclear Matrix - ultrastructure
Nuclear Proteins - analysis
Nuclear Proteins - chemistry
NUCLEOLE
Nucleolin
NUCLEOLOS
NUCLEOLUS
NUCLEOPROTEINAS
NUCLEOPROTEINE
NUCLEOPROTEINS
Phosphoproteins - analysis
Phosphoproteins - chemistry
Plant Proteins - analysis
RACINE
RAICES
RNA-Binding Proteins
ROOTS
ULTRAESTRUCTURA
ULTRASTRUCTURE
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Description
Summary:The analysis of isolated nucleolar matrices from onion cells by light and electron microscopy, 2-D separation of proteins, and confocal microscopy has confirmed the existence of an organized nucleolar matrix with a complex protein composition to which are attached the insoluble processing complexes. In the present work, we present evidence from immunoblotting, immunofluorescence, immunogold labeling, and preferential cytochemical staining with bismuth salts that an insoluble fraction of the multifunctional protein nucleolin, is a component of the onion nucleolar matrix, and analyse its ultrastructural distribution in the described domains of the matrix.
ISSN:0014-4827
1090-2422
DOI:10.1006/excr.1996.0022