Migration of Arterial Wall Cells: Expression of Plasminogen Activators and Inhibitors in Injured Rat Arteries

The expression of plasminogen activators and inhibitors was examined in denuded arteries. Within 5 days, smooth muscle cells (SMCs) on the luminal surface expressed the mRNA for tissue-type plasminogen activator (TPA), urokinase-type plasminogen activator (UPA), the receptor for UPA (UPAR), and plas...

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Bibliographic Details
Published in:Circulation research 1996-03, Vol.78 (3), p.405-414
Main Authors: Reidy, Michael A, Irvin, Colleen, Lindner, Volkhard
Format: Article
Language:English
Subjects:
Animals
Aorta - metabolism
Aorta - pathology
Base Sequence
Biological and medical sciences
Blood vessels and receptors
Cell Movement
DNA, Complementary
Endothelium, Vascular - metabolism
Endothelium, Vascular - pathology
Fundamental and applied biological sciences. Psychology
In Situ Hybridization
Male
Molecular Sequence Data
Plasminogen Activators - biosynthesis
Plasminogen Inactivators - biosynthesis
Rats
Rats, Sprague-Dawley
Receptors, Cell Surface - analysis
Receptors, Urokinase Plasminogen Activator
RNA, Messenger - biosynthesis
Vertebrates: cardiovascular system
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Summary:The expression of plasminogen activators and inhibitors was examined in denuded arteries. Within 5 days, smooth muscle cells (SMCs) on the luminal surface expressed the mRNA for tissue-type plasminogen activator (TPA), urokinase-type plasminogen activator (UPA), the receptor for UPA (UPAR), and plasminogen activator inhibitor type-1 (PAI-1). Similar results were seen after 8 days. Six weeks later, only TPA mRNA was still expressed by SMCs on the luminal surface. En face casein zymograms revealed a net fibrinolytic activity in areas covered with luminal SMCs. Reverse zymography showed no antifibrinolytic activity in these zones. Quiescent endothelial cells did not express TPA, UPA, UPAR, or PAI-1 mRNA. Regenerating endothelium at the wound edge strongly expressed TPA, UPA, and UPAR, as well as PAI-1. UPA and UPAR expression was highly restricted to cells at the wound edge and was not present elsewhere. En face zymography showed no plasmin activity in endothelialized areas, and reverse zymography showed a net antifibrinolytic activity in endothelialized zones. These results suggest that plasminogen activator and inhibitor expression correlates with the migration of both SMCs and endothelial cells into an arterial wound.(Circ Res. 1996;78:405-414)
ISSN:0009-7330
1524-4571
DOI:10.1161/01.RES.78.3.405