Highly sensitive immunoradiometric assay for serum thyroglobulin with minimal interference from autoantibodies

Five monoclonal antibodies (MAbs) directed against antigenic domains on thyroglobulin (Tg) not recognized by most anti-Tg human autoantibodies (aAbs) have been used to develop an improved IRMA for serum Tg with a limit of detection of 0.2 micrograms/L. Samples are incubated for 3 h in tubes coated w...

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Bibliographic Details
Published in:Clinical chemistry (Baltimore, Md.) Md.), 1996-02, Vol.42 (2), p.258-262
Main Authors: Marquet, PY, Daver, A, Sapin, R, Bridgi, B, Muratet, JP, Hartmann, DJ, Paolucci, F, Pau, B
Format: Article
Language:English
Subjects:
Animals
Antibodies, Monoclonal
Autoantibodies - blood
Biological and medical sciences
Endocrinopathies
Humans
Immunoradiometric Assay - methods
Malignant tumors
Medical sciences
Mice
Neoplasm Metastasis
Reagent Kits, Diagnostic
Reproducibility of Results
ROC Curve
Sensitivity and Specificity
Thyroglobulin - blood
Thyroid Neoplasms - blood
Thyroid Neoplasms - radiotherapy
Thyroid Neoplasms - surgery
Thyroid. Thyroid axis (diseases)
Thyroidectomy
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Summary:Five monoclonal antibodies (MAbs) directed against antigenic domains on thyroglobulin (Tg) not recognized by most anti-Tg human autoantibodies (aAbs) have been used to develop an improved IRMA for serum Tg with a limit of detection of 0.2 micrograms/L. Samples are incubated for 3 h in tubes coated with four anti-Tg MAbs. After washing, the tubes are incubated with the tracer MAb for 20 h at room temperature. Dilution and reproducibility tests demonstrated assay reliability. Tests performed on samples with (n = 361) or without (n = 283) aAbs showed that the TG IRMA Pasteur is largely independent of the marked interference generally caused by aAbs. These results were confirmed with an extended population of 2759 samples. For a cutoff of 1 micrograms/L, sensitivity and specificity were 0.97 and 1, respectively, in a follow-up of differentiated thyroid carcinoma in patients treated by total thyroidectomy.
ISSN:0009-9147
1530-8561
DOI:10.1093/clinchem/42.2.258