Isolation of bone marrow plasma cells by negative selection with immunomagnetic beads

In order to isolate bone marrow plasma cells from patients presenting with multiple myeloma or monoclonal gammopathy of undetermined significance, we developed a method for purifying these cells by negative selection using monoclonal antibodies and immunomagnetic beads. The results presented here we...

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Bibliographic Details
Published in:Journal of immunological methods 1996-03, Vol.190 (1), p.127-131
Main Authors: Fillola, Geneviève, Müller, Catherine, Bousquet, Raymonde, Fontanilles, Anne-Marie, Laharrague, Patrick, Corberand, Joël X.
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal
Biological and medical sciences
Bone Marrow Cells
Cell isolation
Cell purification
Cell Separation - methods
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Humans
Immunomagnetic Separation - methods
Molecular immunology
Monoclonal gammopathy of undetermined significance
Monoclonal Gammopathy of Undetermined Significance - pathology
Multiple myeloma
Multiple Myeloma - pathology
Myeloma cell
Plasma cell
Plasma Cells - cytology
Plasma Cells - immunology
Techniques
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Summary:In order to isolate bone marrow plasma cells from patients presenting with multiple myeloma or monoclonal gammopathy of undetermined significance, we developed a method for purifying these cells by negative selection using monoclonal antibodies and immunomagnetic beads. The results presented here were obtained from 75 procedures. Purity was extremely variable (20–100%) and was dependent on the percentage of plasma cells in the original bone marrow sample with a 10% cut-off, beyond which purity was over 96% in all cases. The mean yield was about 20%. The cells collected were viable and suitable for immunophenotyping, semi-quantitative studies of oncoproteins, and PCR.
ISSN:0022-1759
1872-7905
DOI:10.1016/0022-1759(95)00269-3