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Isolation of bone marrow plasma cells by negative selection with immunomagnetic beads

In order to isolate bone marrow plasma cells from patients presenting with multiple myeloma or monoclonal gammopathy of undetermined significance, we developed a method for purifying these cells by negative selection using monoclonal antibodies and immunomagnetic beads. The results presented here we...

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Published in:	Journal of immunological methods 1996-03, Vol.190 (1), p.127-131
Main Authors:	Fillola, Geneviève, Müller, Catherine, Bousquet, Raymonde, Fontanilles, Anne-Marie, Laharrague, Patrick, Corberand, Joël X.
Format:	Article
Language:	English
Subjects:	Antibodies, Monoclonal Biological and medical sciences Bone Marrow Cells Cell isolation Cell purification Cell Separation - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunomagnetic Separation - methods Molecular immunology Monoclonal gammopathy of undetermined significance Monoclonal Gammopathy of Undetermined Significance - pathology Multiple myeloma Multiple Myeloma - pathology Myeloma cell Plasma cell Plasma Cells - cytology Plasma Cells - immunology Techniques
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creator	Fillola, Geneviève Müller, Catherine Bousquet, Raymonde Fontanilles, Anne-Marie Laharrague, Patrick Corberand, Joël X.
description	In order to isolate bone marrow plasma cells from patients presenting with multiple myeloma or monoclonal gammopathy of undetermined significance, we developed a method for purifying these cells by negative selection using monoclonal antibodies and immunomagnetic beads. The results presented here were obtained from 75 procedures. Purity was extremely variable (20–100%) and was dependent on the percentage of plasma cells in the original bone marrow sample with a 10% cut-off, beyond which purity was over 96% in all cases. The mean yield was about 20%. The cells collected were viable and suitable for immunophenotyping, semi-quantitative studies of oncoproteins, and PCR.
doi_str_mv	10.1016/0022-1759(95)00269-3
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Psychology ; Fundamental immunology ; Humans ; Immunomagnetic Separation - methods ; Molecular immunology ; Monoclonal gammopathy of undetermined significance ; Monoclonal Gammopathy of Undetermined Significance - pathology ; Multiple myeloma ; Multiple Myeloma - pathology ; Myeloma cell ; Plasma cell ; Plasma Cells - cytology ; Plasma Cells - immunology ; Techniques</subject><ispartof>Journal of immunological methods, 1996-03, Vol.190 (1), p.127-131</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-e52304acb1158d239d94c2b0daff7130e4785b734eae682067fc56896465032c3</citedby><cites>FETCH-LOGICAL-c417t-e52304acb1158d239d94c2b0daff7130e4785b734eae682067fc56896465032c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3048618$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8601705$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fillola, Geneviève</creatorcontrib><creatorcontrib>Müller, Catherine</creatorcontrib><creatorcontrib>Bousquet, Raymonde</creatorcontrib><creatorcontrib>Fontanilles, Anne-Marie</creatorcontrib><creatorcontrib>Laharrague, Patrick</creatorcontrib><creatorcontrib>Corberand, Joël X.</creatorcontrib><title>Isolation of bone marrow plasma cells by negative selection with immunomagnetic beads</title><title>Journal of immunological methods</title><addtitle>J Immunol Methods</addtitle><description>In order to isolate bone marrow plasma cells from patients presenting with multiple myeloma or monoclonal gammopathy of undetermined significance, we developed a method for purifying these cells by negative selection using monoclonal antibodies and immunomagnetic beads. 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Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunomagnetic Separation - methods</subject><subject>Molecular immunology</subject><subject>Monoclonal gammopathy of undetermined significance</subject><subject>Monoclonal Gammopathy of Undetermined Significance - pathology</subject><subject>Multiple myeloma</subject><subject>Multiple Myeloma - pathology</subject><subject>Myeloma cell</subject><subject>Plasma cell</subject><subject>Plasma Cells - cytology</subject><subject>Plasma Cells - immunology</subject><subject>Techniques</subject><issn>0022-1759</issn><issn>1872-7905</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkE1r3DAQhkVpSDdp_0ELOpSSHJyOJEuyLoES8rEQyKU5C1kepyq2tZW8Cfvvo80ue2xOwzDP-zI8hHxlcMGAqZ8AnFdMS3Nm5HlZlKnEB7JgjeaVNiA_ksUB-UROcv4LAAwUHJPjRgHTIBfkcZnj4OYQJxp72sYJ6ehSii90Nbg8OupxGDJtN3TCp8I9I804oH9LvIT5Dw3juJ7i6J4mnIOnLboufyZHvRsyftnPU_J4c_376q66f7hdXv26r3zN9Fyh5AJq51vGZNNxYTpTe95C5_peMwFY60a2WtToUDUclO69VI1RtZIguBen5Meud5XivzXm2Y4hbz92E8Z1tlobZQRn74JMaqM10wWsd6BPMeeEvV2lUIxsLAO71W63Tu3WqTXSvmm3osS-7fvX7YjdIbT3XO7f93eXvRv65CYf8gErFhrFmoJd7jAs0p4DJpt9wMljF1JxbrsY_v_HKw33nWQ</recordid><startdate>19960328</startdate><enddate>19960328</enddate><creator>Fillola, Geneviève</creator><creator>Müller, Catherine</creator><creator>Bousquet, Raymonde</creator><creator>Fontanilles, Anne-Marie</creator><creator>Laharrague, Patrick</creator><creator>Corberand, Joël X.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19960328</creationdate><title>Isolation of bone marrow plasma cells by negative selection with immunomagnetic beads</title><author>Fillola, Geneviève ; Müller, Catherine ; Bousquet, Raymonde ; Fontanilles, Anne-Marie ; Laharrague, Patrick ; Corberand, Joël X.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-e52304acb1158d239d94c2b0daff7130e4785b734eae682067fc56896465032c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Antibodies, Monoclonal</topic><topic>Biological and medical sciences</topic><topic>Bone Marrow Cells</topic><topic>Cell isolation</topic><topic>Cell purification</topic><topic>Cell Separation - methods</topic><topic>Fundamental and applied biological sciences. 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source	ScienceDirect Journals
subjects	Antibodies, Monoclonal Biological and medical sciences Bone Marrow Cells Cell isolation Cell purification Cell Separation - methods Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunomagnetic Separation - methods Molecular immunology Monoclonal gammopathy of undetermined significance Monoclonal Gammopathy of Undetermined Significance - pathology Multiple myeloma Multiple Myeloma - pathology Myeloma cell Plasma cell Plasma Cells - cytology Plasma Cells - immunology Techniques
title	Isolation of bone marrow plasma cells by negative selection with immunomagnetic beads
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