In Vivo Gene Delivery and Stable Transduction of Nondividing Cells by a Lentiviral Vector

A retroviral vector system based on the human immunodeficiency virus (HIV) was developed that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additio...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1996-04, Vol.272 (5259), p.263-267
Main Authors: Naldini, Luigi, Blömer, Ulrike, Gallay, Philippe, Ory, Daniel, Mulligan, Richard, Gage, Fred H., Verma, Inder M., Trono, Didier
Format: Article
Language:English
Subjects:
AIDS/HIV
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Biotechnology
Brain - cytology
Brain - virology
Cell Division
Cells
Cells, Cultured
Cellular biology
Coding
Female
Fundamental and applied biological sciences. Psychology
Gene therapy
Gene Transfer Techniques
Genetic Therapy
Genetic Vectors
Health. Pharmaceutical industry
HeLa Cells
HIV
HIV - genetics
HIV - physiology
Human immunodeficiency virus
Humans
Industrial applications and implications. Economical aspects
Infections
lentivirus
Macrophages
Macrophages - cytology
Macrophages - virology
Molecular Sequence Data
Neurons - cytology
Neurons - virology
Packaging
Plasmids
Rats
Stem cells
T lymphocytes
Transcripts (Written Records)
Transfection
Virus Integration
Viruses
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Items that cite this one
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Description
Summary:A retroviral vector system based on the human immunodeficiency virus (HIV) was developed that, in contrast to a murine leukemia virus-based counterpart, transduced heterologous sequences into HeLa cells and rat fibroblasts blocked in the cell cycle, as well as into human primary macrophages. Additionally, the HIV vector could mediate stable in vivo gene transfer into terminally differentiated neurons. The ability of HIV-based viral vectors to deliver genes in vivo into nondividing cells could increase the applicability of retroviral vectors in human gene therapy.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.272.5259.263