Gene Expression of Pancreatic Glutamic Acid Decarboxylase in the Nonobese Diabetic Mouse

The 65 kDa isoform of glutamic acid decarboxylase (GAD 65) is an autoantigen implicated in type I diabetes. We have developed a quantitative PCR method to measure GAD 65 mRNA in the pancreas of nonobese diabetic (NOD) mice. Two other nonautoimmune mouse strains were used as controls. In all mice, pa...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-03, Vol.220 (2), p.399-404
Main Authors: Pleau, Jean-Marie, Esling, Anne, Bach, Jean-François, Dardenne, Mireille
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Diabetes Mellitus, Type 1 - enzymology
Diabetes Mellitus, Type 1 - genetics
Female
Gene Expression
Glutamate Decarboxylase - genetics
Glutamate Decarboxylase - metabolism
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred NOD
Molecular Sequence Data
Pancreas - enzymology
Polymerase Chain Reaction
RNA, Messenger - genetics
RNA, Messenger - metabolism
Sex Characteristics
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Summary:The 65 kDa isoform of glutamic acid decarboxylase (GAD 65) is an autoantigen implicated in type I diabetes. We have developed a quantitative PCR method to measure GAD 65 mRNA in the pancreas of nonobese diabetic (NOD) mice. Two other nonautoimmune mouse strains were used as controls. In all mice, pancreatic GAD 65 expression declined with age. In 5-week-old NOD mice, a clear difference was detected between males and females. This sexual dimorphism may explain the absence of tolerance to GAD 65 in the NOD female which could contribute to autoimmune destruction of β cells in the pancreas and subsequent development of diabetes.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0417