Changes in cellular components of spleen and lymph node cells and the effector cells responsible for meth A tumor eradication induced by zinostatin stimalamer

• Published in: Cancer research (Chicago, Ill.) Ill.), 1996-04, Vol.56 (8), p.1868-1873
• Main Authors: MASUDA, E, MAEDA, H
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal; Antigens, Ly - analysis; Antineoplastic agents; B-Lymphocytes - drug effects; B-Lymphocytes - immunology; B-Lymphocytes - pathology; Biological and medical sciences; Chemotherapy; Female; Fibrosarcoma - drug therapy; Fibrosarcoma - immunology; Fibrosarcoma - pathology; Immunophenotyping; Killer Cells, Natural - drug effects; Killer Cells, Natural - immunology; Killer Cells, Natural - pathology; Lymph Nodes - drug effects; Lymph Nodes - immunology; Lymph Nodes - pathology; Lymphocyte Depletion; Macrophages - drug effects; Macrophages - immunology; Macrophages - pathology; Maleic Anhydrides - therapeutic use; Medical sciences; Mice; Mice, Inbred BALB C; Pharmacology. Drug treatments; Polystyrenes - therapeutic use; Spleen - drug effects; Spleen - immunology; Spleen - pathology; T-Lymphocytes - drug effects; T-Lymphocytes - immunology; T-Lymphocytes - pathology; Thy-1 Antigens - analysis; Time Factors; Zinostatin - analogs & derivatives; Zinostatin - therapeutic use
• ISSN: 0008-5472; 1538-7445

We reported previously that pretreatment with zinostatin stimalamer (ZSS) eradicated Meth A tumors in BALB/c mice. We herein investigated cellular components of spleen and lymph node cells of Meth A-bearing ZSS-pretreated mice by flow cytometry; the antitumor effector cells by in vivo depletion of T cells, NK cells, or macrophages; and host-mediated antitumor activity associated with ZSS treatment after tumor transplantation. ZSS given on day-3 transiently decreased the number of spleen cells. The percentage of T cells increased, but B cells and macrophages decreased. B cells decreased in inguinal lymph nodes in Meth A-bearing ZSS-pretreated mice, but increased in Meth A-bearing control mice. In vivo depletion experiments using antibodies or carrageenan showed that antitumor effector cells for tumor eradication are Thy1.2+/Lyt2.2+ and that at least a part of them are asialo GM1+. Thy1.2+/Lyt2.2+/asialoGM1- cells are important in generation of the antitumor activity of ZSS; however, L3T4+ T cells are also involved in initiation of tumor eradication. The result of ZSS treatment after tumor transplantation suggests that ZSS might exhibit antitumor activity by augementating host-mediated antitumor resistance, as well as its intrinsic cytocidal activity.