Identification of differentially expressed genes induced in pancreatic islet neogenesis

Cellophane wrapping of the hamster pancreas induces islet neogenesis. We have used the mRNA differential display technique to select for genes expressed during islet neogenesis but not in control pancreata. Ten candidate clones have been identified. Upon sequencing, 6 clones showed a high degree of...

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Bibliographic Details
Published in:FEBS letters 1996-01, Vol.378 (3), p.219-223
Main Authors: Rafaeloff, Ronit, Qin, Xiao F., Barlow, Scott W., Rosenberg, Lawrence, Vinik, Aaron I.
Format: Article
Language:English
Subjects:
Animals
Blotting, Northern
Cellophane
Cellophane wrap
Cricetinae
Gene Expression
Hamster
In Vitro Techniques
Islet neogenesis
Islets of Langerhans - physiology
mRNA differential display
Pancreas - physiology
Polymerase Chain Reaction - methods
Regeneration - genetics
RNA, Messenger - analysis
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
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Summary:Cellophane wrapping of the hamster pancreas induces islet neogenesis. We have used the mRNA differential display technique to select for genes expressed during islet neogenesis but not in control pancreata. Ten candidate clones have been identified. Upon sequencing, 6 clones showed a high degree of homology to known genes, 1 showed some, and 3 showed no homology to genes of known sequence. Thus, mRNA differential display is a useful technique to identify genes induced during islet neogenesis, and in combination with screening hamster pancreatic cDNA libraries for full length clones, will enhance the likelihood of capturing the participants in this process.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(95)01457-8