Regulation of PHO4 nuclear localization by the PHO80-PHO85 cyclin-CDK complex

PHO4, a transcription factor required for induction of the PHO5 gene in response to phosphate starvation, is phosphorylated by the PHO80-PHO85 cyclin-CDK (cyclin-dependent kinase) complex when yeast are grown in phosphate-rich medium. PHO4 was shown to be concentrated in the nucleus when yeast were...

Full description

Saved in:
Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1996-01, Vol.271 (5246), p.209-212
Main Authors: O'NEILL, E. M, KAFFMAN, A, JOLLY, E. R, O'SHEA, E. K
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Biochemistry
Biological and medical sciences
Cell Nucleus - metabolism
Classical genetics, quantitative genetics, hybrids
Culture Media
Cyclin-Dependent Kinases - metabolism
Cyclins - metabolism
Cytoplasm - metabolism
Dipeptides - metabolism
DNA-Binding Proteins
Fundamental and applied biological sciences. Psychology
Fungal Proteins - metabolism
Gene Expression Regulation, Fungal
Genetic regulation
Genetic transcription
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Logical Thinking
Membrane Transport Proteins - genetics
Molecular Sequence Data
Mutation
Observation
Phosphate Transport Proteins
Phosphates
Phosphates - metabolism
Phosphorylation
Physiological aspects
Regulation
Repressor Proteins
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
Thallophyta, bryophyta
Transcription (Genetics)
Transcription Factors - metabolism
Vegetals
Yeast
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:PHO4, a transcription factor required for induction of the PHO5 gene in response to phosphate starvation, is phosphorylated by the PHO80-PHO85 cyclin-CDK (cyclin-dependent kinase) complex when yeast are grown in phosphate-rich medium. PHO4 was shown to be concentrated in the nucleus when yeast were starved for phosphate and was predominantly cytoplasmic when yeast were grown in phosphate-rich medium. The sites of phosphorylation on PHO4 were identified, and phosphorylation was shown to be required for full repression of PHO5 transcription when yeast were grown in high phosphate. Thus, phosphorylation of PHO4 by PHO80-PHO85 turns off PHO5 transcription by regulating the nuclear localization of PHO4.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.271.5246.209