Evaluation of molecular typing techniques to assign genetic diversity among Saccharomyces cerevisiae strains

Discrimination of strains within the species Saccharomyces cerevisiae was demonstrated by the use of four different techniques to type 15 strains isolated from spoiled wine and beer. Random amplified polymorphic DNA with specific oligonucleotides and PCR fingerprinting with the microsatellite oligon...

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Bibliographic Details
Published in:Applied and Environmental Microbiology 1996-01, Vol.62 (1), p.41-46
Main Authors: Baleiras Couto, M.M. (TNO Nutrition and Food Research, Zeist, The Netherlands.), Eijsma, B, Hofstra, H, Huis in't Veld, J.H.J, Vossen, J.M.B.M. van der
Format: Article
Language:English
Subjects:
ADN
AMPLIFICATION CHAINE POLYMERASE
Base Sequence
Beer
Beer - microbiology
BIERE
Biological and medical sciences
CERVEZAS
DETERIORATION
DETERIORO
DNA Fingerprinting
DNA, Fungal - genetics
DNA, Ribosomal - genetics
EMPREINTE ADN
Food industries
Food microbiology
Fundamental and applied biological sciences. Psychology
Genetic Variation
Genetics
HUELLAS GENETICAS ADN
IDENTIFICACION
IDENTIFICATION
MARCADORES GENETICOS
MARQUEUR GENETIQUE
Microbiology
Molecular biology
Molecular Sequence Data
Mycological methods and techniques used in mycology
Mycological Typing Techniques
Mycology
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
REACCION DE CADENAS DE POLIMERASA
RIBOSOMAS
RIBOSOME
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - classification
Saccharomyces cerevisiae - genetics
Species Specificity
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
VIN
VINOS
Wine - microbiology
Wines
Yeast
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Description
Summary:Discrimination of strains within the species Saccharomyces cerevisiae was demonstrated by the use of four different techniques to type 15 strains isolated from spoiled wine and beer. Random amplified polymorphic DNA with specific oligonucleotides and PCR fingerprinting with the microsatellite oligonucleotide primers (GAC)5 and (GTG)5 enabled discrimination between the strains tested. Additionally, restriction enzyme analysis, with TaqI and MseI of PCR-amplified fragments from the complete internal transcribed spacer and nontranscribed spacer, both present in the rRNA-encoding gene cluster, proved to be suitable for generating intraspecies-specific patterns. Random amplified polymorphic DNA with primers 24 and OPA-11 and PCR fingerprinting with primer (GTG)5 appeared to generate the highest degree of diversity. However, the results indicated that there was no single PCR-mediated typing technique enabling discrimination on the strain level. Discrimination of each individual strain was nevertheless possible by combining the results obtained with all typing techniques
ISSN:0099-2240
1098-5336
DOI:10.1128/aem.62.1.41-46.1996