An immunohistochemical method for detecting bradyzoite antigen (BAG5) in Toxoplasma gondii-infected tissues cross-reacts with a Neospora caninum bradyzoite antigen

The previously cloned gene of a bradyzoite-specific antigen (BAG5) of Toxoplasma gondii was used to express a fusion protein for subsequent antiserum production in rabbits. The BAG5 antiserum was used in an immunohistochemical procedure to look for reactive epitopes in bradyzoites and tachyzoites of...

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Bibliographic Details
Published in:The Journal of parasitology 1996-04, Vol.82 (2), p.354-355
Main Authors: McAllister, M.M. (University of Wyoming, Laramie, WY.), Parmley, S.F, Weiss, L.M, Welch, V.J, McGuire, A.M
Format: Article
Language:English
Subjects:
Animals
Antibodies
ANTIGEN ANTIBODY REACTIONS
ANTIGENE
ANTIGENOS
ANTIGENS
Antigens, Protozoan - analysis
Antigens, Protozoan - immunology
Antiserum
Biological and medical sciences
Bradyzoites
Brain
Brain - parasitology
CHEMISTRY
CHIMIE
Coccidiosis - parasitology
CROSS REACTION
Cross Reactions
Cysts
DETECTION
DEVELOPMENTAL STAGES
Encephalitis
EPITOPES
Epitopes - analysis
Epitopes - immunology
ETAPAS DE DESARROLLO
Experimental protozoal diseases and models
Ferrets
Heart - parasitology
Immune Sera - immunology
Immunoenzyme Techniques
IMMUNOHISTOCHEMISTRY
IMMUNOLOGIE
IMMUNOLOGY
Infections
Infectious diseases
INMUNOLOGIA
Liver
Liver - parasitology
Lung - parasitology
Medical sciences
Mice
Mice, Inbred CBA
Mice, Inbred ICR
Neospora - immunology
NEOSPORA CANINUM
Parasitic diseases
Protozoal diseases
QUIMICA
Rabbits
REACCION ANTIGENO ANTICUERPO
REACTION ANTIGENE ANTICORPS
Research Notes
STADE DE DEVELOPPEMENT
TACHYZOITES
Toxoplasma - immunology
TOXOPLASMA GONDII
Toxoplasmosis, Animal - parasitology
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Description
Summary:The previously cloned gene of a bradyzoite-specific antigen (BAG5) of Toxoplasma gondii was used to express a fusion protein for subsequent antiserum production in rabbits. The BAG5 antiserum was used in an immunohistochemical procedure to look for reactive epitopes in bradyzoites and tachyzoites of T. gondii within animal tissues. Encysted bradyzoites in brain were stained deeply and diffusely. Although most unencysted organisms in brain were not stained, occasional free organisms had mild to deep staining. There was no staining of tachyzoites in liver where cysts were not observed. Neospora caninum organisms within animal tissues were also examined using the BAG5 immunohistochemical procedure. The BAG5 antiserum cross-reacted with N. caninum bradyzoites but had no affinity for tachyzoites.
ISSN:0022-3395
1937-2345
DOI:10.2307/3284181