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Strand Displacement Amplification and the Polymerase Chain Reaction for Monitoring Response to Treatment in Patients with Pulmonary Tuberculosis

Specific amplification of Mycobacterium tuberculosis DNA was investigated as an alternative to conventional microbiologic follow-up in 31 cases of smear- and culture-positive pulmonary tuberculosis. Strand displacement amplification (SDA) and the polymerase chain reaction (PCR) were applied to 438 s...

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Published in:	The Journal of infectious diseases 1996-04, Vol.173 (4), p.934-941
Main Authors:	Hellyer, Tobin J., Fletcher, Terry W., Bates, Joseph H., Stead, William W., Templeton, Gary L., Cave, M. Donald, Eisenach, Kathleen D.
Format:	Article
Language:	English
Subjects:	Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Base Sequence Biological and medical sciences Chemotherapy DNA DNA Primers - chemistry DNA Transposable Elements DNA, Bacterial - analysis Genomes Humans Major Articles Medical sciences Molecular Sequence Data Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Pharmacology. Drug treatments Polymerase chain reaction Polymerase Chain Reaction - methods Predisposing factors Pulmonary tuberculosis Specimens Sputum Sputum - microbiology Tuberculosis Tuberculosis, Pulmonary - diagnosis
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container_title	The Journal of infectious diseases
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creator	Hellyer, Tobin J. Fletcher, Terry W. Bates, Joseph H. Stead, William W. Templeton, Gary L. Cave, M. Donald Eisenach, Kathleen D.
description	Specific amplification of Mycobacterium tuberculosis DNA was investigated as an alternative to conventional microbiologic follow-up in 31 cases of smear- and culture-positive pulmonary tuberculosis. Strand displacement amplification (SDA) and the polymerase chain reaction (PCR) were applied to 438 sequential sputum specimens: 67 (15%) were positive by culture, 248 (57%) by SDA, and 231 (53%) by PCR (χ2 = 3.94, P = .05). Of 200 specimens collected > 180 days after treatment started, none yielded positive cultures, while 50 (25%), representing 16 patients, were positive by both DNA assays. A weak correlation was demonstrated between DNA persistence in sputum and duration of culture positivity (r = 0.45, P = .01), although no correlation was found with the radiographic extent of disease. The inability to distinguish live and dead organisms precludes DNA amplification from use in therapeutic monitoring. For this purpose, quantitative RNA assays are needed if such techniques are to supplant conventional microbiology.
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Donald</creatorcontrib><creatorcontrib>Eisenach, Kathleen D.</creatorcontrib><title>Strand Displacement Amplification and the Polymerase Chain Reaction for Monitoring Response to Treatment in Patients with Pulmonary Tuberculosis</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>Specific amplification of Mycobacterium tuberculosis DNA was investigated as an alternative to conventional microbiologic follow-up in 31 cases of smear- and culture-positive pulmonary tuberculosis. Strand displacement amplification (SDA) and the polymerase chain reaction (PCR) were applied to 438 sequential sputum specimens: 67 (15%) were positive by culture, 248 (57%) by SDA, and 231 (53%) by PCR (χ2 = 3.94, P = .05). Of 200 specimens collected > 180 days after treatment started, none yielded positive cultures, while 50 (25%), representing 16 patients, were positive by both DNA assays. 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Antiparasitic agents</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chemotherapy</subject><subject>DNA</subject><subject>DNA Primers - chemistry</subject><subject>DNA Transposable Elements</subject><subject>DNA, Bacterial - analysis</subject><subject>Genomes</subject><subject>Humans</subject><subject>Major Articles</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Pharmacology. Drug treatments</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Predisposing factors</subject><subject>Pulmonary tuberculosis</subject><subject>Specimens</subject><subject>Sputum</subject><subject>Sputum - microbiology</subject><subject>Tuberculosis</subject><subject>Tuberculosis, Pulmonary - diagnosis</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkUFv0zAYhi0EGmVw54LkA-KWzs4X28lx6hhDKlBBkRAXy3Ec6pHYme0I9i_4ybhrGdw42fLzfq_86UHoOSVLSho4s67vbDyjApbVsoHqAVpQBqLgnMJDtCCkLAtaN81j9CTGa0JIBVycoJOaE2hEtUC_PqWgXIcvbJwGpc1oXMLn4zTY3mqVrHd4j9PO4I0fbkcTVDR4tVPW4Y9G6btE7wN-551NPlj3Lb_HybscSx5vg1HprjQPbHJhvkb8w6Yd3szD6J0Kt3g7tyboefDRxqfoUa-GaJ4dz1P0-fL1dnVVrD-8ebs6Xxe6YjwVLWtVyQRokRepedfRujSKdz2oqin7hgFTJQCUUAtRa0VY1zFmNFDatlXN4BS9OvROwd_MJiY52qjNMChn_BylEI0QwP4fpIwTwVmTg-QQ1MHHGEwvp2DHvJ-kRO5tyYMtmW3JSmZbeeTFsXtuR9PdDxz1ZP7yyFXUauizKp0L_sSAQMVr8rfmOmYF_2AKJWv2PysO3MZkft5zFb5LLkAwefXlqyzX7PKCb6l8D78BV2G5lg</recordid><startdate>19960401</startdate><enddate>19960401</enddate><creator>Hellyer, Tobin J.</creator><creator>Fletcher, Terry W.</creator><creator>Bates, Joseph H.</creator><creator>Stead, William W.</creator><creator>Templeton, Gary L.</creator><creator>Cave, M. 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subjects	Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Base Sequence Biological and medical sciences Chemotherapy DNA DNA Primers - chemistry DNA Transposable Elements DNA, Bacterial - analysis Genomes Humans Major Articles Medical sciences Molecular Sequence Data Mycobacterium tuberculosis Mycobacterium tuberculosis - genetics Pharmacology. Drug treatments Polymerase chain reaction Polymerase Chain Reaction - methods Predisposing factors Pulmonary tuberculosis Specimens Sputum Sputum - microbiology Tuberculosis Tuberculosis, Pulmonary - diagnosis
title	Strand Displacement Amplification and the Polymerase Chain Reaction for Monitoring Response to Treatment in Patients with Pulmonary Tuberculosis
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