Differential Regulation of Inducible Nitric Oxide Synthase Production in Bovine and Caprine Macrophages

Inducible nitric oxide synthase (iNOS) regulation in human and murine macrophages in vitro differs considerably. In this study, expression of macrophage iNOS in ruminants was addressed. Nitric oxide (NO) output by cattle and goat macrophages was as different as that by human and mouse macrophages. B...

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Bibliographic Details
Published in:The Journal of infectious diseases 1996-04, Vol.173 (4), p.971-978
Main Authors: Adler, Heiko, Adler, Barbara, Peveri, Paola, Werner, Ernst R., Wachter, Helmut, Peterhans, Ernst, Jungi, Thomas W.
Format: Article
Language:English
Subjects:
Animals
Antimicrobials
Bacteriophages
Base Sequence
Biological and medical sciences
Cattle
DNA Primers - chemistry
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gene Expression
Goats
Immunobiology
Macrophage Activation
Macrophages
Macrophages - enzymology
Major Articles
Messenger RNA
Mice
Molecular Sequence Data
Monocytes, macrophages
Myeloid cells: ontogeny, maturation, markers, receptors
Nitrate Reductase
Nitrate Reductases - metabolism
Nitrates
Nitrates - metabolism
Nitric Oxide Synthase - metabolism
Nitrites
Oxides
RNA, Messenger - genetics
Salmonella dublin
Ungulates
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Summary:Inducible nitric oxide synthase (iNOS) regulation in human and murine macrophages in vitro differs considerably. In this study, expression of macrophage iNOS in ruminants was addressed. Nitric oxide (NO) output by cattle and goat macrophages was as different as that by human and mouse macrophages. Bovine macrophages activated by heated Salmonella dublin or lipopolysaccharide (LPS) expressed high levels of iNOS mRNA, protein, and enzyme activity. Analogously cultured caprine macrophages did not respond to these and other activators by NO generation and iNOS expression. The lack of response was not due to general unresponsiveness to stimuli. Caprine iNOS mRNA was induced by stimulation of caprine macrophages with LPS, as shown by reverse transcription polymerase chain reaction. The level of mRNA expression in activated goat macrophages was lower than in resting bovine macrophages. A caprine 372-bp iNOS mRNA fragment that was sequenced closely resembled the bovine counterpart. This points to species-specific iNOS gene regulation.
ISSN:0022-1899
1537-6613
DOI:10.1093/infdis/173.4.971