Molecular Cloning, cDNA Structure, and Chromosomal Localization of the Human Type II cGMP-Dependent Protein Kinase

• Published in: Biochemical and biophysical research communications 1996-03, Vol.220 (3), p.759-765
• Main Authors: Ørstavik, S, Solberg, R, Taskén, K, Nordahl, M, Altherr, M.R, Hansson, V, Jahnsen, T, Sandberg, M
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Base Sequence; Cerebellum - enzymology; Chromosome Mapping; Chromosomes, Human, Pair 4; Cloning, Molecular; Colon - enzymology; Cyclic GMP-Dependent Protein Kinases - biosynthesis; Cyclic GMP-Dependent Protein Kinases - genetics; DNA Primers; DNA, Complementary; Female; Gene Library; Humans; Hybrid Cells; Intestine, Small - enzymology; Isoenzymes - biosynthesis; Isoenzymes - genetics; Male; Mice; Molecular Sequence Data; Organ Specificity; Ovary - enzymology; Polymerase Chain Reaction; Prostate - enzymology; RNA, Messenger - analysis; RNA, Messenger - biosynthesis
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1996.0477

The type II cGMP-dependent protein kinase is an enzyme originally isolated from the small intestine, and is thought to be involved in the regulation of intestinal ion transport and fluid secretion. A complementary DNA clone encoding a part of the human type II cGMP-dependent protein kinase was isolated from a cerebellum library. Based on sequence information from this complementary DNA, the 5′-end of the type II cGMP-dependent protein kinase was amplified from human brain messenger RNA using polymerase chain reaction. The composite complementary DNA encoded a 762 amino acid protein with a calculated molecular mass of 87.4 kDa. Messenger RNAs encoding the type II cGMP-dependent protein kinase were detected in small intestine, colon and prostate. By using polymerase chain reaction and Southern blotting on somatic cell hybrids, the gene encoding the type II cGMP-dependent protein kinase was mapped to human chromosome 4 q13.1-q21.1.