Induction of the Adipose Differentiation-Related Protein in Liver of Etomoxir-Treated Rats

The effects of etomoxir, an irreversible carnitine palmitoyltransferase I inhibitor, on the liver protein pattern and on liver morphology were examined by two-dimensional gel electrophoresis in female Sprague-Dawley rats treated with 125 mg/kg/day etomoxir for 28 days. In livers of treated animals a...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-01, Vol.218 (3), p.777-782
Main Authors: Steiner, Sandra, Wahl, Daniel, Mangold, Bonnie L.K., Robison, Rodney, Raymackers, Jos, Meheus, Lydie, Anderson, N.Leigh, Cordier, André
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Carnitine O-Palmitoyltransferase - antagonists & inhibitors
Electrophoresis, Gel, Two-Dimensional
Enzyme Inhibitors - pharmacology
Epoxy Compounds - pharmacology
Female
Liver - drug effects
Liver - metabolism
Liver - pathology
Membrane Proteins - metabolism
Molecular Sequence Data
Peptides - chemistry
Perilipin-2
Rats
Rats, Sprague-Dawley
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Summary:The effects of etomoxir, an irreversible carnitine palmitoyltransferase I inhibitor, on the liver protein pattern and on liver morphology were examined by two-dimensional gel electrophoresis in female Sprague-Dawley rats treated with 125 mg/kg/day etomoxir for 28 days. In livers of treated animals a protein spot was found which was not present in controls. The spot was identified by internal amino acid sequence analysis as the adipose differentiation-related protein (ADRP). The expression of ADRP in liver is a novel finding as the protein has been described previously as adipocyte-specific. Additionally we found histopathologic evidence of lipid accumulation in the livers of etomoxir rats. The data show that for each treated rat there was a good correlation between ADRP levels and degree of lipid droplet formation. This observation may suggest a potential relationship between drug-induced expression of ADRP in liver and lipid accumulation.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.0138