Vigilin contains a functional nuclear localisation sequence and is present in both the cytoplasm and the nucleus

Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH 2-terminal region of phage T7 RNA polymerase as a reporter protein and by s...

Full description

Saved in:
Bibliographic Details
Published in:FEBS letters 1996-03, Vol.382 (3), p.330-334
Main Authors: Kügler, S., Grünweller, A., Probst, C., Klinger, M., Müller, P.K., Kruse, C.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Bacteriophage T7 - enzymology
Carrier Proteins
Cell Line
Cell Nucleus - chemistry
Chickens
Cloning, Molecular
Cytoplasm - chemistry
DNA-Directed RNA Polymerases - genetics
Genes, Reporter - genetics
KH domain
Liver - chemistry
Molecular Sequence Data
Nucleocytoplasmic shuttle
Protein Structure, Secondary
Proteins - analysis
Proteins - chemistry
Proteins - genetics
Rats
Recombinant Fusion Proteins - analysis
RNA-Binding Proteins
Subcellular localization
Vigilin
Viral Proteins
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Vigilin is a member of the KH protein family and contains 14 tandemly arranged potential RNA-binding domains. Between KH domains 2 and 3 we have identified a nuclear localization sequence by cloning this sequence into the NH 2-terminal region of phage T7 RNA polymerase as a reporter protein and by showing its transfer into the nucleus. Furthermore we provide experimental evidence that Vigilin is present both in the nucleus and in the cytoplasm in similar concentrations. These observations support the notion that Vigilin may shuttle between nucleus and cytoplasm presumably in contact with RNA molecules.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(96)00204-9